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Thermomonas fusca sp. nov. and Thermomonas brevis sp. nov., two mesophilic species isolated from a denitrification reactor with poly(-caprolactone) plastic granules as fixed bed, and emended description of the genus Thermomonas

Joris Mergaert, Margo C. Cnockaert and Jean Swings

Laboratorium voor Microbiologie, Vakgroep Biochemie, Fysiologie en Microbiologie, Universiteit Gent, KL Ledeganckstraat 35, B-9000 Gent, Belgium

Correspondence
Jean Swings
jean.swings{at}ugent.be

Previously, 22 aerobic Gram-negative bacteria were isolated from biofilms growing on granules of the synthetic polyester poly(-caprolactone); the granules were used as a fixed bed in a denitrification reactor. All the strains showed similar fatty acid profiles. The 16S rRNA gene sequences of five strains were phylogenetically related to Thermomonas spp. Repetitive extragenic palindromic DNA-PCR (REP-PCR) fingerprinting revealed four groups, and DNA hybridizations between representative strains showed that the strains belonged to two new species within the genus Thermomonas, for which the names Thermomonas fusca (type strain LMG 21737^T=DSM 15424^T) and Thermomonas brevis (type strain LMG 21746^T=DSM 15422^T) are proposed. Both species are able to grow at low temperatures, but not at 50 °C, and are non-haemolytic. Both species can be differentiated by several other phenotypic features from earlier described species of the genus Thermomonas. Cell extracts contain mainly branched fatty acids, with C_{15 : 0} iso, C_{17 : 1} iso 9c, C_{11 : 0} iso 3OH and C_{11 : 0} iso as main constituents. The G+C content of the DNA of the novel species is between 67·6 and 68·7 mol%.

Published online ahead of print on 23 May 2003 as DOI 10.1099/ijs.0.02684-0.

The EMBL accession numbers for the 16S rRNA gene sequences of Thermomonas fusca LMG 21736, LMG 21737^T, LMG 21738 and LMG 21739 and Thermomonas brevis LMG 21746^T are AJ519985, AJ519986, AJ519987, AJ519988 and AJ519989, respectively.

Present address: Louis Parentstraat 7, B-8301 Knokke-Heist, Belgium.

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