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Brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain

¹ Institut für Mikrobiologie und Genetik, Universität Wien, A-1030 Wien, Austria
² DSMZ – Deutsche Sammlung für Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany
³ Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
⁴ Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria

Correspondence
Hans-Jürgen Busse
Hans-Juergen.Busse{at}vu-wien.ac.at

A coccoid- to ovoid-shaped, Gram-positive bacterial strain, designated C3H-21^T, was isolated from the liver of the laboratory mouse strain C3H/He and characterized by a polyphasic approach. The peptidoglycan type was variation A4 with meso-diaminopimelic acid as the diagnostic cell-wall diamino acid and an interpeptide bridge of D-asp-D-Glu. The isolate contained menaquinone MK-7 (88 %) as the major component of the quinone system and minor amounts of menaquinone MK-8 (9 %) and menaquinone MK-6 (3 %). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, unidentified glycolipids and unidentified phospholipids. The fatty acid profile contained predominantly anteiso-C_{15 : 0} and significant amounts of iso-C_{16 : 0}, iso-C_{14 : 0,} anteiso-C_{17 : 0} and C_{19 : 0}. The polyamine pattern consisted of spermine and spermidine as the major compounds. Genomic fingerprints clearly distinguished strain C3H-21^T from other Brachybacterium species. The isolate shared the highest 16S rDNA sequence similarities with members of the genus Brachybacterium, in particular Brachybacterium sacelli LMG 20345^T, Brachybacterium nesterenkovii DSM 9573^T, Brachybacterium rhamnosum LMG 19848^T, Brachybacterium alimentarium CNRZ 925^T and Brachybacterium fresconis LMG 20336^T (97·8–97·2 %). The results of biochemical/physiological characterization, chemotaxonomic characteristics and REP-PCR-generated fingerprints demonstrated that the isolate represents a novel species of the genus Brachybacterium, for which the name Brachybacterium muris (type strain C3H-21^T=DSM 14560^T=CCM 7047^T) is proposed.

Published online ahead of print on 6 June 2003 as DOI 10.1099/ijs.0.02728-0.

The EMBL accession number for the 16S rDNA sequence of Brachybacterium muris C3H-21^T is AJ537574.

An image showing the REP-PCR fingerprints of B. muris C3H-21^T and related Brachybacterium spp. is available in IJSEM Online.

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