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Int J Syst Evol Microbiol 53 (2003), 1941-1946; DOI  10.1099/ijs.0.02666-0
© 2003 International Union of Microbiological Societies

rDNA sequence analyses of Streptococcus dysgalactiae subsp. equisimilis isolates from pigs

Koji Kawata1, Taichi Minakami1, Yukari Mori1, Masanori Katsumi2, Yasushi Kataoka3, Ayako Ezawa1, Naoya Kikuchi1 and Tatsuhumi Takahashi1

1 Department of Epizootiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan
2 Sendai Meat Inspection Center, Sendai, Miyagi 983, Japan
3 Department of Veterinary Microbiology, Nippon Veterinary and Animal Science University, Musashino, Tokyo 180, Japan

Correspondence
Koji Kawata
kawako{at}rakuno.ac.jp

The nucleotide sequences of 16S and 23S rRNA genes (rDNA) were determined for 11 isolates of Streptococcus dysgalactiae subsp. equisimilis from slaughtered pigs with endocarditis, arthritis or lymphadenitis and strain ATCC 35666, designated as a strain of subspecies equisimilis. The sequences of each of the genes were compared phylogenetically with the corresponding sequences of S. dysgalactiae subsp. dysgalactiae ATCC 43078T and ATCC 27957, which were also determined in this study. Based on the 16S rDNA analysis, the isolates of S. dysgalactiae subsp. equisimilis were divided into two distinct groups, designated groups 1 and 2. S. dysgalactiae subsp. equisimilis ATCC 35666 was closely related to the group 2 strains. The S. dysgalactiae subsp. dysgalactiae strains seemed to be associated with the group 1 strains, which was not consistent with the conventional subspecific classification of S. dysgalactiae. In contrast, the 23S rDNA analysis distinguished S. dysgalactiae subsp. dysgalactiae strains from subsp. equisimilis strains. This inconsistency between phylogenies based on 16S and 23S rDNA indicates that 23S rDNA is a more rigid marker for determining the phylogenetic relationships and taxonomic position of these organisms than is 16S rDNA.

Published online ahead of print on 20 June 2003 as DOI 10.1099/ijs.0.02666-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S and 23S rDNA sequences determined in this paper are given in Fig. 2.






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