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Idiomarina loihiensis sp. nov., a halophilic -Proteobacterium from the L‘ihi submarine volcano, Hawai‘i

Stuart P. Donachie¹, Shaobin Hou¹, Todd S. Gregory^2,, Alexander Malahoff^2,3 and Maqsudul Alam¹

¹ Department of Microbiology, University of Hawai‘i, Snyder Hall III, 2538 The Mall, Honolulu, HI 96822, USA
² Department of Ocean and Resources Engineering, SOEST, University of Hawai‘i, Holmes Hall, 2540 Dole Street, Honolulu, HI 96822, USA
³ Institute of Geological and Nuclear Sciences, PO Box 30368, 69 Gracefield Road, Lower Hutt, New Zealand

Correspondence
Maqsudul Alam
alam{at}hawaii.edu

During an investigation of bacterial diversity at hydrothermal vents on the L‘ihi Seamount, Hawai‘i, a novel bacterium (designated L2-TR^T) was cultivated, which shares 99·9 % 16S rRNA gene sequence similarity over 1415 nt with an uncultured eubacterium from sediment at a depth of 11 000 m in the Mariana Trench. The nearest cultivated neighbour of L2-TR^T, however, is Idiomarina abyssalis KMM 227^T, with which it shares 98·9 % 16S rRNA sequence similarity. L2-TR^T differed from I. abyssalis KMM 227^T in several phenotypic respects, including growth at 46 °C and in medium that contained 20 % (w/v) NaCl. DNA–DNA hybridization data showed that L2-TR^T did not belong to the species I. abyssalis (43·4 % DNA–DNA reassociation). Cells of L2-TR^T were Gram-negative rods, 0·35 µm wide and 0·7–1·0 µm long, which were occasionally up to 1·8 µm in length. Cells were motile by a single polar or subpolar flagellum. The major fatty acid in L2-TR^T was iso-C_{15 : 0} (32·6 %). The DNA G+C content was 47·4 mol%. Phenotypic and genotypic analyses indicated that L2-TR^T could be assigned to the genus Idiomarina but, based on significant phenotypic and genotypic differences, constituted a novel species within this genus, Idiomarina loihiensis sp. nov., of which L2-TR^T (=ATCC BAA-735^T=DSM 15497^T) is the type strain.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of L2-TR^T is AF288370.

Details of the growth of Idiomarina loihiensis sp. nov. in different NaCl concentrations and its full fatty acid composition are available as supplementary material in IJSEM Online.

Present address: Institute for Exploration, WHOI, Mail Stop #7, Woods Hole, MA 02543, USA.

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