| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Clinical Microbiology, University Hospital, SE-751 85 Uppsala, Sweden
2 Department of Molecular Evolution, EBC, Uppsala University, Norbyvägen 18C, SE-195 30 Uppsala, Sweden
Correspondence
Björn Herrmann
bjorn.herrmann{at}medsci.uu.se
The rnpB gene is universally present in bacterial species and encodes the RNA subunit of endoribonuclease P. In this study, rnpB was sequenced in 50 type strains and 29 additional strains of the genus Streptococcus. Putative secondary-structure models and possible interactions in RNase P RNA molecules are discussed. Phylogenetic relationships were studied and Bayesian, maximum-parsimony and minimum-evolution analyses supported six main clades that comprised 22 of the 50 species analysed. Phylogenetic inference was also studied for the 16S rRNA gene; it indicated a similar tree topology, but with weaker support values than for rnpB. Combined analysis of rnpB and 16S resulted in a phylogeny with significantly better support. Variability in the rnpB and 16S genes among all type strains, calculated as Shannon–Wiener information index values, was 0·45 for rnpB and 0·15 for 16S. Intraspecies proximity was assessed by principal coordinate analysis of rnpB for 32 strains of six closely related species (two clades) and showed species-specific clusters, but heterogeneity occurred in two species. It can be concluded that the rnpB gene is suitable for phylogenetic analysis of closely related taxa and has potential as a tool for species discrimination.
Published online ahead of print on 16 May 2003 as DOI 10.1099/ijs.0.02639-0.
The GenBank/EMBL/DDBJ accession numbers for rnpB are listed in Table 1.
This article has been cited by other articles:
|
|
 |
|
  O. O. Glazunova, D. Raoult, and V. Roux Partial sequence comparison of the rpoB, sodA, groEL and gyrB genes within the genus Streptococcus Int J Syst Evol Microbiol, September 1, 2009; 59(9): 2317 - 2322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Tappe, R. Pukall, P. Schumann, S. Gronow, M. Spiliotis, H. Claus, K. Brehm, and U. Vogel Streptococcus merionis sp. nov., isolated from Mongolian jirds (Meriones unguiculatus) Int J Syst Evol Microbiol, April 1, 2009; 59(4): 766 - 770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. R. Peters, C. R. Helps, L. McAuliffe, H. Neimark, M. R. Lappin, T. J. Gruffydd-Jones, M. J. Day, L. E. Hoelzle, B. Willi, M. Meli, et al. RNase P RNA Gene (rnpB) Phylogeny of Hemoplasmas and Other Mycoplasma Species J. Clin. Microbiol., May 1, 2008; 46(5): 1873 - 1877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Davies, D. J. McMillan, G. H. Van Domselaar, M. K. Jones, and K. S. Sriprakash Phage 3396 from a Streptococcus dysgalactiae subsp. equisimilis Pathovar May Have Its Origins in Streptococcus pyogenes J. Bacteriol., April 1, 2007; 189(7): 2646 - 2652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Haanpera, J. Jalava, P. Huovinen, O. Meurman, and K. Rantakokko-Jalava Identification of Alpha-Hemolytic Streptococci by Pyrosequencing the 16S rRNA Gene and by Use of VITEK 2 J. Clin. Microbiol., March 1, 2007; 45(3): 762 - 770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Innings, M. Ullberg, A. Johansson, C. J. Rubin, N. Noreus, M. Isaksson, and B. Herrmann Multiplex Real-Time PCR Targeting the RNase P RNA Gene for Detection and Identification of Candida Species in Blood J. Clin. Microbiol., March 1, 2007; 45(3): 874 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wan, S. L. Broschat, and D. R. Call Validation of Mixed-Genome Microarrays as a Method for Genetic Discrimination Appl. Envir. Microbiol., March 1, 2007; 73(5): 1425 - 1432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Innings, M. Krabbe, M. Ullberg, and B. Herrmann Identification of 43 Streptococcus Species by Pyrosequencing Analysis of the rnpB Gene J. Clin. Microbiol., December 1, 2005; 43(12): 5983 - 5991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-J. Rubin, M. Thollesson, L. A. Kirsebom, and B. Herrmann Phylogenetic relationships and species differentiation of 39 Legionella species by sequence determination of the RNase P RNA gene rnpB Int J Syst Evol Microbiol, September 1, 2005; 55(5): 2039 - 2049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. N. Zadoks, Y. H. Schukken, and M. Wiedmann Multilocus Sequence Typing of Streptococcus uberis Provides Sensitive and Epidemiologically Relevant Subtype Information and Reveals Positive Selection in the Virulence Gene pauA J. Clin. Microbiol., May 1, 2005; 43(5): 2407 - 2417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Hassan, O. Akineden, and E. Usleber Identification of Streptococcus canis Isolated from Milk of Dairy Cows with Subclinical Mastitis J. Clin. Microbiol., March 1, 2005; 43(3): 1234 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. LI and S. ALTMAN In search of RNase P RNA from microbial genomes RNA, October 20, 2004; 10(10): 1533 - 1540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Picard, D. Ke, D. K. Boudreau, M. Boissinot, A. Huletsky, D. Richard, M. Ouellette, P. H. Roy, and M. G. Bergeron Use of tuf Sequences for Genus-Specific PCR Detection and Phylogenetic Analysis of 28 Streptococcal Species J. Clin. Microbiol., August 1, 2004; 42(8): 3686 - 3695. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
