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Int J Syst Evol Microbiol 53 (2003), 1665-1670; DOI  10.1099/ijs.0.02470-0
© 2003 International Union of Microbiological Societies

Metschnikowia vanudenii sp. nov. and Metschnikowia lachancei sp. nov., from flowers and associated insects in North America

Gabriella Giménez-Jurado1, Cletus P. Kurtzman2, William T. Starmer3 and Isabel Spencer-Martins1

1 Centro de Recursos Microbiológicos (CREM), Biotechnology Unit, Faculty of Sciences and Technology, New University of Lisbon, 2829-516 Caparica, Portugal
2 National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA
3 Department of Biology, Syracuse University, NY 13244, USA

Correspondence
Isabel Spencer-Martins
ism{at}fct.unl.pt

Two new species of the ascosporic yeast genus Metschnikowia were isolated from nectaries and associated muscoid flies of flowers from the common milkweed (Asclepias syriaca) in North America, and are described as Metschnikowia vanudenii [type strain=PYCC 4650T=CBS 9134T=NRRL Y-27243T=UWO(PS) 86A4.1T] and Metschnikowia lachancei [type strain=PYCC 4605T=CBS 9131T=NRRL Y-27242T=UWO(PS) 7ASB2.3T]. As with the previously described Metschnikowia gruessii, M. vanudenii has vegetative cells with an ‘aeroplane’ or cross-like configuration, produces ovoid chlamydospores and forms ellipsoidopedunculate asci with two acicular ascospores. Metschnikowia lachancei is distinguished from other Metschnikowia species by formation of club-shaped asci with 1–2 thick clavate ascospores. The phylogenetic positions of the proposed new species within Metschnikowia were determined from sequence analysis of the D1/D2 domain of 26S rDNA. The new species show low nuclear DNA relatedness with neighbouring taxa.


Abbreviations: nDNA, nuclear DNA

Published online ahead of print on 11 April 2003 as DOI 10.1099/ijs.0.02470-0.

The GenBank accession numbers for the D1/D2 26S rDNA sequences of strain PYCC 4650T and PYCC 4605T are AF017404 and AY080995, respectively.




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