| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
ek2
í Do
ka2
vec1ek1
1 Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic
2 Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Kotláská 2, 611 37 Brno, Czech Republic
3 BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium
Correspondence
Ivo Sedláek
ivo{at}sci.muni.cz
Eight strains of Gram-positive, catalase- and oxidase-positive cocci were isolated from the skin of llamas (Lama glama L.) and characterized using a polyphasic approach. These strains were assigned to the genus Macrococcus on the basis of their phenotypic properties (resistance to bacitracin and sensitivity to furazolidone) and DNA base content (40–42 mol% G+C). Phylogenetic analysis based on 16S rDNA confirmed that the strains are members of the genus Macrococcus. They differed from all hitherto described macrococcal species in their production of phosphatase and reduction of nitrate (most strains) and the inability to produce acid from glycerol or to grow in 7·5 % NaCl. Ribotyping (EcoRI), macrorestriction analysis (XbaI) and fatty acid methyl ester analysis divided the strains from llamas into three stable clusters. Moreover, ribotyping differentiated the strains analysed not only from previously described macrococcal species but also from oxidase-positive staphylococci. DNA–DNA hybridization confirmed that the three clusters represent separate genomic groups (similarity values<54 %). All the results showed that the strains represent three novel species, for which the names Macrococcus hajekii sp. nov. (type strain CCM 4809T=LMG 21711T), Macrococcus brunensis sp. nov. (type strain CCM 4811T=LMG 21712T) and Macrococcus lamae sp. nov. (type strain CCM 4815T=LMG 21713T) are proposed.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of M. hajekii CCM 4809T, M. brunensis CCM 4811T and M. lamae CCM 4815T are respectively AY119685–AY119687.
This article has been cited by other articles:
|
|
 |
|
  T. Baba, K. Kuwahara-Arai, I. Uchiyama, F. Takeuchi, T. Ito, and K. Hiramatsu Complete Genome Sequence of Macrococcus caseolyticus Strain JSCS5402, Reflecting the Ancestral Genome of the Human-Pathogenic Staphylococci J. Bacteriol., February 15, 2009; 191(4): 1180 - 1190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Novakova, I. Sedlacek, R. Pantucek, V. Stetina, P. Svec, and P. Petras Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens. J. Med. Microbiol., May 1, 2006; 55(Pt 5): 523 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Pantucek, I. Sedlacek, P. Petras, D. Koukalova, P. Svec, V. Stetina, M. Vancanneyt, L. Chrastinova, J. Vokurkova, V. Ruzickova, et al. Staphylococcus simiae sp. nov., isolated from South American squirrel monkeys Int J Syst Evol Microbiol, September 1, 2005; 55(5): 1953 - 1958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Svec, M. Vancanneyt, L. A. Devriese, S. M. Naser, C. Snauwaert, K. Lefebvre, B. Hoste, and J. Swings Enterococcus aquimarinus sp. nov., isolated from sea water Int J Syst Evol Microbiol, September 1, 2005; 55(5): 2183 - 2187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Svec, M. Vancanneyt, I. Sedlacek, K. Engelbeen, V. Stetina, J. Swings, and P. Petras Reclassification of Staphylococcus pulvereri Zakrzewska-Czerwinska et al. 1995 as a later synonym of Staphylococcus vitulinus Webster et al. 1994 Int J Syst Evol Microbiol, November 1, 2004; 54(6): 2213 - 2215. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
