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Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas

Sylva Mannerová¹, Roman Pantek², Jií Doka², Pavel vec¹, Cindy Snauwaert³, Marc Vancanneyt³, Jean Swings³ and Ivo Sedláek¹

¹ Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic
² Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Kotláská 2, 611 37 Brno, Czech Republic
³ BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium

Correspondence
Ivo Sedláek
ivo{at}sci.muni.cz

Eight strains of Gram-positive, catalase- and oxidase-positive cocci were isolated from the skin of llamas (Lama glama L.) and characterized using a polyphasic approach. These strains were assigned to the genus Macrococcus on the basis of their phenotypic properties (resistance to bacitracin and sensitivity to furazolidone) and DNA base content (40–42 mol% G+C). Phylogenetic analysis based on 16S rDNA confirmed that the strains are members of the genus Macrococcus. They differed from all hitherto described macrococcal species in their production of phosphatase and reduction of nitrate (most strains) and the inability to produce acid from glycerol or to grow in 7·5 % NaCl. Ribotyping (EcoRI), macrorestriction analysis (XbaI) and fatty acid methyl ester analysis divided the strains from llamas into three stable clusters. Moreover, ribotyping differentiated the strains analysed not only from previously described macrococcal species but also from oxidase-positive staphylococci. DNA–DNA hybridization confirmed that the three clusters represent separate genomic groups (similarity values<54 %). All the results showed that the strains represent three novel species, for which the names Macrococcus hajekii sp. nov. (type strain CCM 4809^T=LMG 21711^T), Macrococcus brunensis sp. nov. (type strain CCM 4811^T=LMG 21712^T) and Macrococcus lamae sp. nov. (type strain CCM 4815^T=LMG 21713^T) are proposed.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of M. hajekii CCM 4809^T, M. brunensis CCM 4811^T and M. lamae CCM 4815^T are respectively AY119685–AY119687.

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