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1 College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China
2 College of Resource and Environment Sciences, Northwest Science and Technology University of Agriculture and Forestry, Yangling Shaanxi 712100, People's Republic of China
3 Departamento de Microbiología, Escuela Nacional de Ciencias biológicas, Instituto Politécnico Nacional, México, DF, Mexico
Correspondence
Wen Xin Chen
wenxin_chen{at}263.net
Twenty-nine rhizobial isolates from root nodules of Astragalus and Lespedeza spp. growing in the Loess Plateau of China were characterized by numerical taxonomy, RFLP and sequencing of PCR-amplified 16S rRNA genes, measurement of DNA G+C content, DNA–DNA relatedness and cross-nodulation with selected legume species. Based on the results of numerical taxonomy, the isolates formed two clusters (1 and 2) with some single isolates at a similarity level of 82 %. Cluster 1 contained six isolates from Astragalus and Lespedeza spp. Cluster 2 consisted of nine isolates from Astragalus spp. DNA relatedness was greater than 80 % among isolates within cluster 2. Phylogenetic analysis based on 16S rRNA gene sequences showed that CCBAU 7190BT, representing cluster 2, was closely related to Rhizobium galegae and Rhizobium huautlense. DNA–DNA relatedness between CCBAU 7190BT and reference strains of R. galegae, R. huautlense and other related species ranged from 0 to 48·6 %. The cluster 2 isolates could also be differentiated phenotypically from related species. Based on these data, a novel species, Rhizobium loessense sp. nov., is proposed for cluster 2, with the type strain CCBAU 7190BT (=AS1.3401T=LMG 21975T).
The GenBank/EMBL/DDBJ accession number of the 16S rDNA sequence of strain CCBAU 7190BT is AF364069.
Details of the sources of reference strains used in this study and a 16S rDNA-based phylogenetic tree showing a wider selection of reference rhizobial strains are available as supplementary material in IJSEM Online.
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