HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Erratum Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hatano, K. Articles by Kasai, H. Search for Related Content PubMed PubMed Citation Articles by Hatano, K. Articles by Kasai, H. Agricola Articles by Hatano, K. Articles by Kasai, H.

Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA–DNA hybridization and sequences of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Arai 1957) corrig., sp. nov., nom. rev.

Kazunori Hatano^1,, Tadashi Nishii¹ and Hiroaki Kasai²

¹ Institute for Fermentation, Osaka, 17-85, Juso-honmachi 2-chome, Yodogawa-ku, Osaka 532-8686, Japan
² Marine Biotechnology Institute, Kamaishi Laboratories, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan

Correspondence
Kazunori Hatano
hatano-kazunori{at}nite.go.jp

The taxonomic status of 64 strains of whorl-forming Streptomyces (formerly Streptoverticillium) species was re-evaluated and strains were reclassified on the basis of their phenotypes, DNA–DNA hybridization data and partial sequences of gyrB, the structural gene of the B subunit of DNA gyrase. These strains, which consisted of 46 species and eight subspecies with validly published names and 13 species whose names have not been validly published [including 10 strains examined by the International Streptomyces Project (ISP)], were divided into two groups, namely typical and atypical whorl-forming Streptomyces species, based on their phenotypes and gyrB gene sequences. The typical whorl-forming species (59 strains) were divided into six major clusters of three or more species, seven minor clusters of two species and five single-member clusters, based on the threshold value of 97 % gyrB sequence similarity. Major clusters were typified by Streptomyces abikoensis, Streptomyces cinnamoneus, Streptomyces distallicus, Streptomyces griseocarneus, Streptomyces hiroshimensis and Streptomyces netropsis. Phenotypically, members of each cluster resembled each other closely except for the S. distallicus cluster, which was divided phenotypically into the S. distallicus and Streptomyces stramineus subclusters, and the S. netropsis cluster, which was divided into the S. netropsis and Streptomyces eurocidicus subclusters. Strains in each minor cluster closely resembled each other phenotypically. DNA–DNA relatedness between the representative species and others in each major cluster and/or subcluster, and between strains in the minor clusters, was >70 %, indicating that the major clusters and/or subclusters and the minor clusters each comprise a single species. It was concluded that 59 strains of typical whorl-forming Streptomyces species consisted of the following 18 species, including subjective synonym(s): S. abikoensis, Streptomyces ardus, Streptomyces blastmyceticus, S. cinnamoneus, S. eurocidicus, S. griseocarneus, S. hiroshimensis, Streptomyces lilacinus, ‘Streptomyces luteoreticuli’, Streptomyces luteosporeus, Streptomyces mashuensis, Streptomyces mobaraensis, Streptomyces morookaense, S. netropsis, Streptomyces orinoci, S. stramineus, Streptomyces thioluteus and Streptomyces viridiflavus.

The GenBank/EMBL/DDBJ accession numbers for the gyrB gene sequences are shown in Fig. 1.

Present address: Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 2-5-8, Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.

This article has been cited by other articles:

				T. Clavel, C. Charrier, A. Braune, M. Wenning, M. Blaut, and D. Haller Isolation of bacteria from the ileal mucosa of TNFdeltaARE mice and description of Enterorhabdus mucosicola gen. nov., sp. nov. Int J Syst Evol Microbiol, July 1, 2009; 59(7): 1805 - 1812. [Abstract] [Full Text] [PDF]

				P. Schumann, P. Kampfer, H.-J. Busse, L. I. Evtushenko, and for the Subcommittee on the Taxonomy of the Subord Proposed minimal standards for describing new genera and species of the suborder Micrococcineae Int J Syst Evol Microbiol, July 1, 2009; 59(7): 1823 - 1849. [Abstract] [Full Text] [PDF]

				X. Wu, Y. Wen, C. Qian, O. Li, H. Fang, and W. Chen Taxonomic study of a chromomycin-producing strain and reclassification of Streptomyces cavourensis subsp. washingtonensis as a later synonym of Streptomyces griseus Int J Syst Evol Microbiol, December 1, 2008; 58(12): 2783 - 2787. [Abstract] [Full Text] [PDF]

				Y. Guo, W. Zheng, X. Rong, and Y. Huang A multilocus phylogeny of the Streptomyces griseus 16S rRNA gene clade: use of multilocus sequence analysis for streptomycete systematics Int J Syst Evol Microbiol, January 1, 2008; 58(1): 149 - 159. [Abstract] [Full Text] [PDF]

				H. Yamamura, T. Tamura, Y. Sakiyama, and S. Harayama Nocardia amamiensis sp. nov., isolated from a sugar-cane field in Japan Int J Syst Evol Microbiol, July 1, 2007; 57(7): 1599 - 1602. [Abstract] [Full Text] [PDF]

				Z. Liu, Y. Shi, Y. Zhang, Z. Zhou, Z. Lu, W. Li, Y. Huang, C. Rodriguez, and M. Goodfellow Classification of Streptomyces griseus (Krainsky 1914) Waksman and Henrici 1948 and related species and the transfer of 'Microstreptospora cinerea' to the genus Streptomyces as Streptomyces yanii sp. nov. Int J Syst Evol Microbiol, July 1, 2005; 55(4): 1605 - 1610. [Abstract] [Full Text] [PDF]

				Y. Huang, W. Li, L. Wang, B. Lanoot, M. Vancanneyt, C. Rodriguez, Z. Liu, J. Swings, and M. Goodfellow Streptomyces glauciniger sp. nov., a novel mesophilic streptomycete isolated from soil in south China Int J Syst Evol Microbiol, November 1, 2004; 54(6): 2085 - 2089. [Abstract] [Full Text] [PDF]