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Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval ‘Virgilkapelle’ in Vienna

Hans-Jürgen Busse^1,2, Christian Zlamala^1,2, Sandra Buczolits^1,2, Werner Lubitz¹, Peter Kämpfer³ and Mariko Takeuchi^4,

¹ Institut für Mikrobiologie und Genetik, Universität Wien, A-1030 Wien, Austria
² Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, Veterinärplatz 1, A-1210 Wien, Austria
³ Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität, Giessen D-35392, Germany
⁴ Institute for Fermentation, Osaka, 17-85 Juso-honmachi 2-chome, Yodogawa-ku, Osaka 532-8686, Japan

Correspondence
Hans-Jürgen Busse
hans-juergen.busse{at}vu-wien.ac.at

Two airborne bacterial isolates designated V45^T and V54A^T were characterized in order to determine their taxonomic position. 16S rDNA sequence analysis showed that the two isolates shared 98·1 % sequence similarity. Highest sequence similarities (98·0–98·5 %) were found to Promicromonospora citrea DSM 43110^T and Promicromonospora sukumoe IFO 14650^T. Detection of a quinone system with the predominant compound MK-9(H₄), a polar lipid pattern containing phosphatidylglycerol, a fatty acid profile with the predominant acids C_{15 : 0} iso and C_{15 : 0} anteiso and the diagnostic cell-wall diamino acid L-lysine supported the assignment of the novel isolates to the genus Promicromonospora. The two isolates could be distinguished from P. sukumoe by the presence of glycine in the peptidoglycan, and the detection of the cell-wall sugar galactose differentiates them from the two established species of the genus Promicromonospora. Each of the two isolates displayed a unique biochemical profile. Results from DNA–DNA hybridizations clearly demonstrated that V45^T and V54A^T represent separate species. Based on these data, it is proposed that V45^T (=IFO 16525^T=CCM 7044^T) and V54A^T (=IFO 16526^T =CCM 7043^T) be classified as the type strains of two novel Promicromonospora species, for which the names Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov. are proposed.

The GenBank/EMBL/DDBJ accession numbers for the 16S rDNA sequences of strains V45^T and V54A^T are AJ487302 and AJ487303.

DNA–DNA hybridization results and micrographs of cells of the novel strains are available as supplementary material in IJSEM Online.

Present address: Takeda Chemical Industries, 17-85, Juso-honmachi 2-chome, Yodogawa-ku, Osaka 532-8686, Japan.

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