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Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedis ‘Haemophilus somnus’, ‘Haemophilus agni’ and ‘Histophilus ovis’

¹ Danish Veterinary Institute, Bülowsvej 27, DK-1790 Copenhagen V, Denmark
² University of Bern, Veterinary Bacteriology, Langgassstrasse 122, CH-3012 Bern, Switzerland
³ Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark
⁴ Institute for Medical Microbiology and Hygiene, Philipps-University, Pilgrimstein 2, D-35037 Marburg, Germany

Correspondence
Øystein Angen
ang{at}vetinst.dk

Earlier investigations have shown that ‘Haemophilus somnus’, ‘Haemophilus agni’ and ‘Histophilus ovis’ represent the same species. In the present investigation, the taxonomic position of this species is explored further by sequencing the 16S rRNA and rpoB genes of strains that were investigated previously by DNA–DNA hybridization. These results clearly support the allocation of this species to a novel genus within the family Pasteurellaceae. The phenotypic separation of Histophilus somni gen. nov., sp. nov. from other members of the family can, for most strains, be based on capnophilia, yellowish pigmentation and indole production. However, due to phenotypic variation, the use of a species-specific PCR test based on the 16S rRNA gene is included in the species description. This is justified by the high sequence similarity of the 16S rRNA gene within the species and the fact that the highest sequence similarity to any other taxon within the family is 93·4 %. The type strain, 8025^T=ATCC 43625^T=CCUG 36157^T, was isolated in the USA from a bovine brain with lesions of thromboembolic meningoencephalitis.

The GenBank/EMBL/DDBJ accession numbers for the 16S rDNA and rpoB sequences reported in this paper are AF549387–AF549405 and AY170207–AY170219, respectively.

The full phylogenetic tree based on 16S rDNA sequences is available as supplementary material in IJSEM Online.

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