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1 Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University, 5-1-1-1 Minamisawa, Minami-ku, Sapporo 005-8601, Japan
2 National Institute of Advanced Industrial Science and Technology, Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan
3 Japan Collection of Microorganisms, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama 351-0198, Japan
4 Research Laboratory, Higeta Shoyu Co., Choshi, Chiba 288-8680, Japan
Correspondence
Hidetoshi Matsuyama
matsuyama{at}db.htokai.ac.jp
A novel strictly aerobic, heterotrophic, mesophilic bacterium, strain Mb8T, was isolated from soil in Sapporo City, Hokkaido, Japan. The G+C content of strain Mb8T was 66·0 mol%. It had mycolic acids with 4452 carbon atoms and C16 : 0 and C18 : 1 (9) as the major fatty acids. The major isoprenoid quinone was MK-8(H2). The cell wall contained meso-diaminopimelic acid, arabinose and galactose. 16S rDNA, chemotaxonomic and morphological data indicated that this strain clearly belonged to the genus Rhodococcus. Based on phenotypic properties and DNADNA hybridization data, strain Mb8T (=JCM 11308T=NCIMB 13903T) has been assigned to the genus Rhodococcus as the type strain of Rhodococcus tukisamuensis sp. nov.
Published online ahead of print on 7 February 2003 as DOI 10.1099/ijs.0.02523-0.
The DDBJ/GenBank/EMBL accession number for the 16S rRNA sequence of strain Mb8T is AB067734.
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