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1 Department of Pediatrics and Communicable Diseases, University of Michigan, 1150 W. Med. Ctr. Dr., MSRB III, Rm 8323, Ann Arbor, MI 48109-0646, USA
2 Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
Correspondence
Tom Coenye
Tom.Coenye{at}UGent.be
Thirteen Ralstonia pickettii-like isolates from the environment (water, soil and activated sludge) and human clinical samples (including respiratory secretions of cystic fibrosis patients) were investigated in a polyphasic taxonomic study that employed 16S rDNA sequence analysis, DNA–DNA hybridization, determination of DNA base composition, whole-cell protein analysis, biochemical characterization and PCR-based assays. All isolates were classified as a novel Ralstonia species, for which the name Ralstonia insidiosa sp. nov. is proposed. The type strain, LMG 21421T (=CCUG 46789T), was isolated from the sputum of a patient with acute lymphoblastic leukaemia. R. insidiosa can be differentiated from other species of the genus Ralstonia and phenotypically similar species (including the Burkholderia cepacia complex and Achromobacter xylosoxidans) by a variety of biochemical tests, whole-cell protein analysis and several PCR-based assays. Some outstanding issues in the taxonomy of the genus Ralstonia are also discussed.
Published online ahead of print on 29 November 2002 as DOI 10.1099/ijs.0.02555-0.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Ralstonia insidiosa LMG 21421T is AF488779.
Figures showing normalized whole-cell protein profiles and PCR analysis with primer pair Rp-F1/R38R1 of Ralstonia strains are available as supplementary material in IJSEM Online.
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