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Int J Syst Evol Microbiol 53 (2003), 1013-1018; DOI  10.1099/ijs.0.02521-0
© 2003 International Union of Microbiological Societies

Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments

Hana Yi1, Young-Hyo Chang2, Hyun Woo Oh2, Kyung Sook Bae2 and Jongsik Chun1

1 School of Biological Sciences, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea
2 Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Yusung PO Box 115, Taejon 305-600, Republic of Korea

Correspondence
Jongsik Chun
jchun{at}snu.ac.kr

Two red pigment-producing bacterial strains with a metallic green sheen were isolated from a sediment sample of getbol, the Korean tidal flat. Phylogenetic analysis based on 16S rDNA sequences showed that these isolates represent a phyletic lineage within the {gamma}-Proteobacteria that is distantly related to the genus Hahella. No bacterial species with validly published names showed >=92 % 16S rRNA similarity with the getbol isolates. The strains were Gram-negative, chemo-organotrophic, aerobic and required NaCl (1–7 %) for growth. They produced pigments with maximum absorption at 540 nm, which indicated the presence of prodigiosin, a well-known red pigment previously detected in Serratia marcescens. The major isoprenoid quinone was ubiquinone-9. The predominant cellular fatty acids were saturated and monounsaturated straight-chain fatty acids. The DNA G+C contents ranged from 40 to 42 mol%. The combination of physiological, biochemical and chemotaxonomic data clearly separated the test strains from other phylogenetically related genera in the {gamma}-Proteobacteria. On the basis of polyphasic evidence from this study, it is proposed that the two getbol isolates should be classified in a novel genus, Zooshikella gen. nov., as Zooshikella ganghwensis sp. nov.


Published online ahead of print on 29 November 2002 as DOI 10.1099/ijs.0.02521-0.

The GenBank accession numbers for the 16S rDNA sequences of strains JC2044T and JC2045 are AY130994 and AY130995, respectively.




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