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Aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in Thailand

¹ Laboratorium voor Microbiologie, Ghent University, K.L. Ledeganckstr. 35, B-9000 Ghent, Belgium
² Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK
³ Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, IFZ, Heinrich-Buff-Ring 26–32, D-35392 Giessen, Germany
⁴ Laboratorium voor BCCM^TM/LMG Bacteria Collection, Ghent University, K.L. Ledeganckstr. 35, B-9000 Ghent, Belgium

Correspondence
Geert Huys
geert.huys{at}rug.ac.be

A group of seven sucrose-negative Aeromonas strains (referred to as group Au) isolated from the internal organs of septicaemic farmed frogs (Rana rugulosa) in Thailand was subjected to a polyphasic taxonomic study including fluorescent amplified fragment length polymorphism (FAFLP) and ERIC-PCR fingerprinting, 16S rDNA sequencing, microplate DNA–DNA hybridizations and extensive phenotypic characterization. Comparison of FAFLP and ERIC-PCR fingerprints indicated that the group Au isolates belonged to the species Aeromonas hydrophila DNA hybridization group (HG) 1 in which they represent a genotypic subgroup closely affiliated to A. hydrophila subsp. hydrophila and subsp. dhakensis. One representative of the Au group exhibited 99·0 % 16S rDNA sequence similarity with the type strains of the two A. hydrophila subspecies. DNA–DNA hybridization with type and reference strains of all known Aeromonas taxa revealed that the Au group represented a homogeneous taxon that exhibited the highest relatedness with members of the two A. hydrophila subspecies, ranging from 75 to 93 %. Phenotypic characterization on the basis of 152 features further revealed that the Au group isolates differed from A. hydrophila subsp. hydrophila or subsp. dhakensis in a total of 13 biochemical properties. Of these, assimilation of L-glycine and isobutyrate as sole carbon source, acid production from salicin and D-sucrose, and aesculin hydrolysis were of diagnostic value. From the results of this study, it can be concluded that the Aeromonas frog isolates of the Au group represent a new subspecies of A. hydrophila, for which the name Aeromonas hydrophila subsp. ranae subsp. nov. is proposed. Its type strain is Au-1D12^T (=LMG 19707^T=CCUG 46211^T).

Published online ahead of print on 29 November 2002 as DOI 10.1099/ijs.0.02357-0.

The EMBL accession number for the 16S rDNA sequence of Au strain LMG 19707^T is AJ508766.

Supplementary tables are available in IJSEM Online.

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