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Amycolatopsis keratiniphila sp. nov., a novel keratinolytic soil actinomycete from Kuwait

¹ Kuwait University, Department of Biological Sciences, Microbiology Division, P.O. Box 5969, 13060 Safat, Kuwait
² DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, 38124 Braunschweig, Germany

Correspondence
Reiner M. Kroppenstedt
kdt{at}dsmz.de

A keratinolytic, nocardioform actinomycete, strain D2^T, was isolated from Kuwait marsh soil by the bait technique using animal wool. Strain D2^T was an aerobic, Gram-positive organism that produced light-grey aerial mycelium but no specific spore chains. 16S rDNA sequence analyses and chemotaxonomic markers were consistent with the classification of strain D2^T in the genus Amycolatopsis, i.e. it had meso-diaminopimelic acid in its peptidoglycan, arabinose and galactose as its diagnostic sugars, the polar lipids phosphatidylinositol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and diphosphatidylglycerol, menaquinone MK-9(H₄) and an iso-/anteiso-branched fatty acid pattern combined with 10-methyl-branched and 2-hydroxy-branched fatty acids. Amycolatopsis japonica was the closest phylogenetic neighbour of strain D2^T, showing 99·4 % 16S rDNA sequence similarity with the novel strain. A. japonica and strain D2^T could be clearly separated from each other on the basis of their low DNA–DNA reassociation value (55·9 %). These data, together with its distinct physiological traits, led to the conclusion that strain D2^T represented a novel species within the genus Amycolatopsis, for which the name Amycolatopsis keratiniphila (type strain D2^T=DSM 44409^T=NRRL B24117^T) is proposed.

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