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Thermococcus gammatolerans sp. nov., a hyperthermophilic archaeon from a deep-sea hydrothermal vent that resists ionizing radiation

Edmond Jolivet^1,, Stéphane L'Haridon¹, Erwan Corre², Patrick Forterre³ and Daniel Prieur¹

¹ UMR 6539, Centre National de la Recherche Scientifique et Université de Bretagne Occidentale, Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzané, France
² UMR 7127, Station Biologique, CNRS et Université Pierre et Marie Curie, Place Georges Teissier, 29682 Roscoff Cedex, France
³ UMR 8621, Institut de Génétique et Microbiologie, CNRS et Université Paris-Sud, Bât 409, 91405 Orsay Cedex, France

Correspondence
Edmond Jolivet
ejolivet{at}lsu.edu

Enrichments for anaerobic organotrophic hyperthermophiles were performed with hydrothermal chimney samples collected at the Guaymas Basin (27° 01' N, 111° 24' W). Positive enrichments were submitted to -irradiation at a dose of 30 kGy. One of the resistant strains, designated strain EJ3^T, formed regular motile cocci. The new strain grew between 55 and 95 °C, with an optimum growth temperature of 88 °C. The optimal pH for growth was 6·0, and the optimum NaCl concentration for growth was around 20 g l^-1. Strain EJ3^T was an obligately anaerobic heterotroph that utilized yeast extract, tryptone and peptone. Elemental sulfur or cystine was required for growth and reduced to hydrogen sulfide. The G+C content of the genomic DNA was 51·3 mol%. As determined by 16S rRNA gene sequence analysis, the organism was most closely related to Thermococcus celer, Thermococcus guaymasensis, Thermococcus hydrothermalis, Thermococcus profundus and Thermococcus gorgonarius. However, no significant homology was observed between them by DNA–DNA hybridization. The novel organism also possessed phenotypic traits that differ from those of its closest phylogenetic relatives. Therefore, it is proposed that this isolate, which constitutes the most radioresistant hyperthermophilic archaeon known to date, should be described as the type strain of a novel species, Thermococcus gammatolerans sp. nov. The type strain is EJ3^T (=DSM 15229^T=JCM 11827^T).

The GenBank accession number for the 16S rRNA sequence of Thermococcus gammatolerans strain EJ3^T is AF479014.

Data on the effect of temperature, pH and NaCl concentration on EJ3^T are available as supplementary material in IJSEM Online.

Present address: Dept of Biological Sciences, Louisiana State University and A & M College, Baton Rouge, LA 70803, USA.

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