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Sulfurihydrogenibium subterraneum gen. nov., sp. nov., from a subsurface hot aquifer

¹ Subground Animalcule Retrieval (SUGAR) Project, Frontier Research System for Extremophiles, Japan Marine Science & Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan
² The DEEPSTAR Group, Frontier Research System for Extremophiles, Japan Marine Science & Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan
³ Department of Earth Sciences, University of Southern California, Los Angeles, CA 90089-0740, USA
⁴ NASA Jet Propulsion Laboratory, 4800 Oak Grove Drive, Pasadena, CA 91109-8099, USA

Correspondence
Ken Takai
kent{at}jamstec.go.jp

A polyphasic taxonomic study was performed on a novel facultatively anaerobic, hydrogen- or sulfur/thiosulfate-oxidizing, thermophilic chemolithoautotroph recently isolated from subsurface hot aquifer water in a Japanese gold mine. The cells were straight to slightly curved rods, with a single polar flagellum. Growth was observed at 40–70 °C (optimum 60–65 °C; 80 min doubling time) and at pH 6·4–8·8 (optimum pH 7·5). The isolate was unable to use complex organic compounds, carbohydrates, amino acids or organic acids as sole energy and carbon sources. The G+C content of the genomic DNA was 31·3 mol%. Phylogenetic analysis based on 16S rDNA sequences indicated that the isolate was closely related to an uncultivated group of micro-organisms within the order Aquificales obtained from Icelandic and Japanese hot spring microbial mats, but distantly related to previously identified genera of the Aquificales such as Persephonella, Aquifex and Hydrogenobacter. The name Sulfurihydrogenibium subterraneum gen. nov., sp. nov. is proposed for this novel species; the type strain is HGMK1^T (=JCM 11477^T=ATCC BAA-562^T=DSM 15120^T).

Published online ahead of print on 25 October 2002 as DOI 10.1099/ijs.0.02506-0.

The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of Sulfurihydrogenibium subterraneum HGMK1^T is AB071324.

Growth curves showing the effects of temperature, pH and sea-salt concentration on growth of Sulfurihydrogenibium subterraneum are available as supplementary material in IJSEM Online.

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