HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hoover, R. B. Articles by Krader, P. Search for Related Content PubMed PubMed Citation Articles by Hoover, R. B. Articles by Krader, P. Agricola Articles by Hoover, R. B. Articles by Krader, P.

Spirochaeta americana sp. nov., a new haloalkaliphilic, obligately anaerobic spirochaete isolated from soda Mono Lake in California

¹ NASA/NSSTC, SD-50, 320 Sparkman Dr., Huntsville, AL 35805, USA
² Department of Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
³ Laboratory for Structural Biology, The University of Alabama in Huntsville, MSB, Huntsville, AL 35899, USA
⁴ Department of Microbiology, University of Georgia, Athens, GA 30602-2605, USA
⁵ American Type Culture Collection, 10801 University Blvd, Manassas, VA 20110, USA

Correspondence
Richard B. Hoover
Richard.B.Hoover{at}NASA.GOV
Elena V. Pikuta
Elena.Pikuta{at}msfc.nasa.gov

A novel, obligately anaerobic, mesophilic, haloalkaliphilic spirochaete, strain ASpG1^T, was isolated from sediments of the alkaline, hypersaline Mono Lake in California, USA. Cells of the Gram-negative strain were motile and spirochaete-shaped with sizes of 0·2–0·22x8–18 µm. Growth of the strain was observed between 10 and 44 °C (optimum 37 °C), in 2–12 % (w/v) NaCl (optimum 3 % NaCl) and between pH 8 and 10·5 (optimum pH 9·5). The novel strain was strictly alkaliphilic, required high concentrations of carbonates in the medium and was capable of utilizing D-glucose, fructose, maltose, sucrose, starch and D-mannitol. End products of glucose fermentation were H₂, acetate, ethanol and formate. Strain ASpG1^T was resistant to kanamycin and rifampicin, but sensitive to gentamicin, tetracycline and chloramphenicol. The G+C content of its DNA was 58·5 mol%. DNA–DNA hybridization analysis of strain ASpG1^T with its most closely related species, Spirochaeta alkalica Z-7491^T, revealed a hybridization value of only 48·7 %. On the basis of its physiological and molecular properties, strain ASpG1^T appears to represent a novel species of the genus Spirochaeta, for which the name Spirochaeta americana is proposed (type strain ASpG1^T=ATCC BAA-392^T=DSM 14872^T).

The GenBank accession number for the 16S rDNA sequence of Spirochaeta americana ASpG1^T is AF373921.

This article has been cited by other articles:

				H. Imachi, S. Sakai, H. Hirayama, S. Nakagawa, T. Nunoura, K. Takai, and K. Horikoshi Exilispira thermophila gen. nov., sp. nov., an anaerobic, thermophilic spirochaete isolated from a deep-sea hydrothermal vent chimney Int J Syst Evol Microbiol, October 1, 2008; 58(10): 2258 - 2265. [Abstract] [Full Text] [PDF]

				E. V. Pikuta, D. Marsic, T. Itoh, A. K. Bej, J. Tang, W. B. Whitman, J. D. Ng, O. K. Garriott, and R. B. Hoover Thermococcus thioreducens sp. nov., a novel hyperthermophilic, obligately sulfur-reducing archaeon from a deep-sea hydrothermal vent Int J Syst Evol Microbiol, July 1, 2007; 57(7): 1612 - 1618. [Abstract] [Full Text] [PDF]

				E. V. Pikuta, T. Itoh, P. Krader, J. Tang, W. B. Whitman, and R. B. Hoover Anaerovirgula multivorans gen. nov., sp. nov., a novel spore-forming, alkaliphilic anaerobe isolated from Owens Lake, California, USA. Int J Syst Evol Microbiol, November 1, 2006; 56(Pt 11): 2623 - 2629. [Abstract] [Full Text] [PDF]

				E. V. Pikuta, R. B. Hoover, A. K. Bej, D. Marsic, W. B. Whitman, P. E. Krader, and J. Tang Trichococcus patagoniensis sp. nov., a facultative anaerobe that grows at -5 {degrees}C, isolated from penguin guano in Chilean Patagonia. Int J Syst Evol Microbiol, September 1, 2006; 56(Pt 9): 2055 - 2062. [Abstract] [Full Text] [PDF]

				W. B. Whitman Error in G+C calculations (Letter) Int J Syst Evol Microbiol, May 1, 2006; 56(5): 1177 - 1177. [Full Text] [PDF]

				E. V. Pikuta, D. Marsic, A. Bej, J. Tang, P. Krader, and R. B. Hoover Carnobacterium pleistocenium sp. nov., a novel psychrotolerant, facultative anaerobe isolated from permafrost of the Fox Tunnel in Alaska Int J Syst Evol Microbiol, January 1, 2005; 55(1): 473 - 478. [Abstract] [Full Text] [PDF]

				E. V. Pikuta, R. B. Hoover, A. K. Bej, D. Marsic, W. B. Whitman, D. Cleland, and P. Krader Desulfonatronum thiodismutans sp. nov., a novel alkaliphilic, sulfate-reducing bacterium capable of lithoautotrophic growth Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1327 - 1332. [Abstract] [Full Text] [PDF]