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Recognition of two genetic groups in the Klebsiella oxytoca taxon on the basis of chromosomal -lactamase and housekeeping gene sequences as well as ERIC-1R PCR typing

¹ Service de Microbiologie-Hygiène, Hôpital Ambroise Paré AP-HP, Université Versailles-Saint-Quentin-en-Yvelines-UFR Médicale Paris-Ile-de-France-Ouest, 9 avenue Charles de Gaulle, 92100 Boulogne-Billancourt, France
² Laboratoire de Microbiologie Médicale, Fondation Hôpital Saint-Joseph, Paris, France
³ Centre de Biologie et d'Ecologie Tropicale et Méditerranéenne, UMR 5555, Université de Perpignan, France
⁴ Service de Microbiologie, Centre Hospitalier du Pays d'Aix, Aix en Provence, France

Correspondence
Marie-Hélène Nicolas-Chanoine
marie-helene.nicolas-chanoine{at}apr.ap-hop-paris.fr

Whilst searching for a molecular method to identify the different species of Raoultella and Klebsiella oxytoca, it was observed that the OXY-1 and OXY-2 -lactamase-producing K. oxytoca isolates displayed two distinguishable enterobacterial repetitive intergenic consensus (ERIC)-1R profiles. It was hypothesized that the two groups of chromosomal -lactamases might correspond to two groups of strains in the K. oxytoca taxon. To confirm this hypothesis, clinical isolates and reference strains of K. oxytoca were studied by determination of the sequence of their bla_OXY genes, and of a partial fragment of their 16S rRNA (387 bp) and rpoB (512 bp) genes. The sequence data were phylogenetically analysed by using the parsimony method. Four clinical isolates possessed a bla_OXY-1 gene and nine possessed a bla_OXY-2 gene. The mean percentage of rpoB and 16S rRNA gene identity was >99 % within each group of strains, whereas it was 96·56±0·24 % for rpoB genes and 97·80±0·22 % for 16S rRNA genes between the group of strains harbouring the bla_OXY-1 gene and the group harbouring the bla_OXY-2 gene. The phylogenetic tree resulting from combined analysis of the 16S rRNA and rpoB datasets showed that the K. oxytoca isolates were monophyletic and separated into two clades; these clades included strains with either the bla_OXY-1 gene or the bla_OXY-2 gene. This result was supported with high bootstrap values of 97 and 99 %, respectively. Moreover, the two groups of strains displayed distinct ERIC-1R profiles, with bands characteristic of each profile. Thus, the chromosomal bla_OXY gene sequence is able to delineate not only two groups of -lactamases in K. oxytoca, but also two clades in the K. oxytoca taxon, in a manner similar to the sequence of housekeeping genes. These results suggest that K. oxytoca should be divided into two genetic groups, group OXY-1 represented by K. oxytoca strain SL781 (=CIP 104963) and group OXY-2 by K. oxytoca strain SL911 (=CIP 106098).

Published online ahead of print on 23 August 2002 as DOI 10.1099/ijs.0.02408-0.

Alignments of consensus DNA sequences of the 16S rRNA and and rpoB genes are available as supplementary data in IJSEM Online.

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