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Mycobacterium shottsii sp. nov., a slowly growing species isolated from Chesapeake Bay striped bass (Morone saxatilis)

¹ Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA
² United States Department of Agriculture, Beltsville, MD, USA
³ Tuberculosis/Mycobacteriology Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA
⁴ US Geological Survey, Leetown Science Center, National Fish Health Research Laboratory, Kearneysville, WV, USA

Correspondence
Martha W. Rhodes
martha{at}vims.edu

Slowly growing, non-pigmented mycobacteria were isolated from striped bass (Morone saxatilis) during an epizootic of mycobacteriosis in the Chesapeake Bay. Growth characteristics, acid-fastness and results of 16S rRNA gene sequencing were consistent with those of the genus Mycobacterium. A unique profile of biochemical reactions was observed among the 21 isolates. A single cluster of eight peaks identified by analysis of mycolic acids (HPLC) resembled those of reference patterns but differed in peak elution times from profiles of reference species of the Mycobacterium tuberculosis complex. One isolate (M175^T) was placed within the slowly growing mycobacteria by analysis of aligned 16S rRNA gene sequences and was proximate in phylogeny to Mycobacterium ulcerans and Mycobacterium marinum. However, distinct nucleotide differences were detected in the 16S rRNA gene sequence among M175^T, M. ulcerans and M. marinum (99·2 % similarity). Isolate M175^T could be differentiated from other slowly growing, non-pigmented mycobacteria by its inability to grow at 37 °C, production of niacin and urease, absence of nitrate reductase and resistance to isoniazid (1 µg ml^-1), thiacetazone and thiophene-2-carboxylic hydrazide. Based upon these genetic and phenotypic differences, isolate M175^T (=ATCC 700981^T =NCTC 13215^T) is proposed as the type strain of a novel species, Mycobacterium shottsii sp. nov.

The GenBank accession number for the 16S rRNA sequence of Mycobacterium shottsii M175^T is AY005147.

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