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Int J Syst Evol Microbiol 53 (2003), 303-307; DOI  10.1099/ijs.0.02350-0
© 2003 International Union of Microbiological Societies


Note

Agromyces aurantiacus sp. nov., isolated from a Chinese primeval forest

Wen-Jun Li1, Li-Ping Zhang1, Ping Xu1, Xiao-Long Cui1, Li-Hua Xu1, Zhongse Zhang1, Peter Schumann2, Erko Stackebrandt2 and Cheng-Lin Jiang1

1 The Key Laboratory for Microbial Resources of Ministry of Education, PR China, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, China
2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany

Correspondence
Cheng-Lin Jiang
lihxu{at}ynu.edu.cn

A catalase-negative actinomycete, strain YIM 21741T, was isolated from a soil sample collected from a primeval forest at Xishuangbanna, Yunnan Province, China. Analysis of 16S rDNA showed the strain to be related to members of the genus Agromyces, with which it also shares morphological characteristics, e.g. branching hyphae breaking into diphtheroid and rod-like, irregular, non-motile fragments and a peptidoglycan containing the diagnostic amino acid 2,4-diamino-n-butyric acid. Whole-cell hydrolysates of strain YIM 21741T contained rhamnose and small quantities of glucose, galactose and mannose. The major menaquinone was MK-12, while MK-13 and MK-12 were minor components. Diagnostic phospholipids were phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the DNA was 72·8 mol%. Physiological and biochemical characteristics reveal strain YIM 21741T to be different from all validly described species of the genus Agromyces. As DNA–DNA similarity values between this isolate and the phylogenetically neighbouring type strains of Agromyces bracchium and Agromyces luteolus are only moderate, the novel species Agromyces aurantiacus sp. nov. is proposed with strain YIM 21741T (=CCTCC 001012T =AS 4.1717T =DSM 14598T) as the type strain.


Abbreviations: DAB, 2,4-diamino-n-butyric acid; ISP, International Streptomyces Project

The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of strain YIM 21741T is AF389342.




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