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Int J Syst Evol Microbiol 53 (2003), 289-293; DOI  10.1099/ijs.0.02441-0
© 2003 International Union of Microbiological Societies

Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases

Philip Hugenholtz{dagger} and Thomas Huber

ComBinE group, Advanced Computational Modelling Centre, The University of Queensland, Brisbane 4072, Australia

Correspondence
Philip Hugenholtz
philiph{at}nature.berkeley.edu

A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.

Published online ahead of print on 26 July 2002 as DOI 10.1099/ijs.0.02441-0.

{dagger}Present address: Environmental Science, Policy and Management, Division of Ecosystem Sciences, 151 Hilgard Hall, University of California Berkeley, Berkeley, CA 94720-3110, USA.




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