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ComBinE group, Advanced Computational Modelling Centre, The University of Queensland, Brisbane 4072, Australia
Correspondence
Philip Hugenholtz
philiph{at}nature.berkeley.edu
A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.
Present address: Environmental Science, Policy and Management, Division of Ecosystem Sciences, 151 Hilgard Hall, University of California Berkeley, Berkeley, CA 94720-3110, USA.
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