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Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are -radiation resistant

¹ Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA
² National Research Institute of Fisheries Science, Food Processing Division, Kanazawa-ku, Yokohama-City, Kanagawa 236-8648, Japan
³ Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA

Correspondence
Kasthuri Venkateswaran
kjvenkat{at}jpl.nasa.gov

One of the spore-formers isolated from a spacecraft-assembly facility, belonging to the genus Bacillus, is described on the basis of phenotypic characterization, 16S rDNA sequence analysis and DNA–DNA hybridization studies. It is a Gram-positive, facultatively anaerobic, rod-shaped eubacterium that produces endospores. The spores of this novel bacterial species exhibited resistance to UV, -radiation, H₂O₂ and desiccation. The 16S rDNA sequence analysis revealed a clear affiliation between this strain and members of the low G+C Firmicutes. High 16S rDNA sequence similarity values were found with members of the genus Bacillus and this was supported by fatty acid profiles. The 16S rDNA sequence similarity between strain FO-92^T and Bacillus benzoevorans DSM 5391^T was very high. However, molecular characterizations employing small-subunit 16S rDNA sequences were at the limits of resolution for the differentiation of species in this genus, but DNA–DNA hybridization data support the proposal of FO-92^T as Bacillus nealsonii sp. nov. (type strain is FO-92^T =ATCC BAA-519^T =DSM 15077^T).

Published online ahead of print on 5 July 2002 as DOI 10.1099/ijs.0.02311-0.

The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of strain FO-92^T is AF234863.

Images of the Jet Propulsion Laboratory Spacecraft Assembly Facility are available as supplementary data in IJSEM Online (http://ijs.sgmjournals.org).

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