International Journal of Systematic and Evolutionary Microbiology, Vol 52, 2113-2120, Copyright © 2002 by Society for General Microbiology

Pseudomonas extremorientalis sp. nov., isolated from a drinking water reservoir

E. P. Ivanova, N. M. Gorshkova, T. Sawabe, K. Hayashi, N. I. Kalinovskaya, A. M. Lysenko, N. V. Zhukova, D. V. Nicolau, T. A. Kuznetsova, V. V. Mikhailov and R. Christen
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russia

On the basis of phenotypic and genotypic characteristics and 16S rDNA sequence analysis, a novel species belonging to the genus Pseudomonas sensu stricto was identified. The saprophytic, fluorescent bacterium, designated KMM 3447(T), was isolated from a drinking water reservoir near Vladivostok City, Russia. The novel organism was a Gram-negative, aerobic, rod-shaped bacterium that produced a cyclic depsipeptide with surface-active properties. It degraded casein, but did not degrade gelatin, starch, agar or Tween 80. The bacterium was also haemolytic. Growth of the novel bacterium occurred between 4 and 35 degrees C. The predominant cellular fatty acids of the novel pseudomonad were C(16:0), C(16:1(n-7)), C(18:1(n-7)) and C(17:0 cyclo); branched fatty acids were only found in trace amounts. The G+C content of the novel bacterium was 61.0 mol%. 16S rDNA sequence analysis indicated that the novel bacterium had a clear affiliation with Pseudomonas fluorescens and species closely related to this recognized pseudomonad. DNA--DNA hybridization experiments showed that the novel bacterium bound at low levels (27--53%) with the DNA of the type strains of its nearest phylogenetic relatives, namely Pseudomonas tolaasii, Pseudomonas veronii, Pseudomonas orientalis and Pseudomonas rhodesiae, indicating that the novel bacterium represented a novel species within the genus Pseudomonas, for which the name Pseudomonas extremorientalis is proposed; the type strain is KMM 3447(T) (=LMG 19695(T)).

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