International Journal of Systematic and Evolutionary Microbiology, Vol 52, 1455-1459, Copyright © 2002 by Society for General Microbiology

Psychromonas marina sp. nov., a novel halophilic, facultatively psychrophilic bacterium isolated from the coast of the Okhotsk Sea

K. Kawasaki, Y. Nogi, M. Hishinuma, Y. Nodasaka, H. Matsuyama and I. Yumoto
Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology, 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan

A facultatively psychrophilic bacterium, strain 4-22(T), was isolated from a cold current off the Monbetsu coast of the Okhotsk Sea in Hokkaido, Japan. The isolate was a rod-shaped facultative anaerobe that reduced nitrate to nitrite and hydrolysed starch, DNA and alginic acid, but not chitin or gelatin. The isolate grew at 0 degrees C, but not at 26 degrees C; the optimum growth temperature was 14--16 degrees C. NaCl was required for growth. The DNA G+C content was 43.5 mol%. The whole-cell fatty acids consisted of significant amounts of an unsaturated fatty acid, C(16:1), and a saturated fatty acid, C(16:0). A polyunsaturated fatty acid, docosahexaenoic acid (C(22:6)), was also detected (1.6%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 4-22(T) was closely related to Psychromonas antarctica (95.7% similarity). DNA--DNA hybridization revealed a relatedness of 31% between strain 4-22(T) and P. antarctica. Based on physiological and biochemical characteristics and the phylogenetic position as determined by 16S rRNA gene analysis and DNA--DNA relatedness, it is concluded that the isolate represents a novel species, for which the name Psychromonas marina sp. nov. is proposed. The type strain is 4-22(T) (=JCM 10501(T)=IAM 14899(T)=NCIMB 13792(T)).

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