International Journal of Systematic and Evolutionary Microbiology, Vol 52, 945-951, Copyright © 2002 by Society for General Microbiology

Comparison between Streptococcus macedonicus and Streptococcus waius strains and reclassification of Streptococcus waius (Flint et al. 1999) as Streptococcus macedonicus (Tsakalidou et al. 1998)

P. L. Manachini, S. H. Flint, LJH. Ward, W. Kelly, M. G. Fortina, C. Parini and D. Mora
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Industriale, University of Milano, Via Celoria 2, 20133 Milano, Italy

Two species of dairy streptococci, Streptococcus waius and Streptococcus macedonicus, were originally characterized by 16S--23S intergenic spacer sequence analysis, random amplified polymorphic DNA fingerprinting, PFGE analysis and DNA--DNA reassociation experiments. All genetic data suggested that S. waius strains belong to the previously described species S. macedonicus. Likewise, the phenotypic characterization showed that strains of S. macedonicus and S. waius were highly related and easily differentiated from the closest phylogenetic neighbour, Streptococcus bovis, principally by their failure to produce a blackening reaction in medium containing aesculin. The utilization of maltose and cellobiose by S. macedonicus/S. waius strains allowed their differentiation from the most studied dairy species, Streptococcus thermophilus. On the basis of genetic and phenotypic data S. macedonicus and S. waius species should be considered synonyms and S. macedonicus has the priority.

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