International Journal of Systematic and Evolutionary Microbiology, Vol 52, 875-881, Copyright © 2002 by Society for General Microbiology

Georgenia muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting

P. Altenburger, P. Kampfer, P. Schumann, D. Vybiral, W. Lubitz and H. J. Busse
Institut fur Bakteriologie, Mykologie und Hygiene, Veterinarmedizinische Universitat, A-1210 Wien, Austria

Two bacterial strains, designated 1A-C(T) and 3A-1, were studied and, using these results and previously published data, taxonomically classified. Cells of the strains exhibited a rod--coccus cycle. The peptidoglycan determined for 1A-C(T) was of type A4alpha with lysine as the diagnostic cell-wall diamino acid and an interpeptide bridge of L-Lys <- L-Glu. The menaquinone systems of the two strains contained MK-8(H(4)) (82--94%) and MK-7(H(4)) (3--11%). The polar lipid profiles consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, two unidentified phospholipids and an unidentified glycolipid. The fatty acid profiles contained predominantly ai-C(15:0) and significant amounts of i-C14:0 and i-C15:1 fatty acids. Genomic fingerprints clearly distinguished strains 1A-C(T) and 3A-1 from each other. DNA--DNA relatedness between the two strains (92%) demonstrated that they are members of a single species. Analyses of the 16S rDNA sequences of strains 1A-C(T) and 3A-1, which were almost identical (99.6% sequence similarity), and comparison with corresponding sequences demonstrated that they represent a novel lineage within the suborder Micrococcineae, most closely related to species of the genera Beutenbergia, Bogoriella and Cellulomonas (94.7--95.7% sequence similarity). The results demonstrate that the two strains are members of a single new genus and a single novel species. Thus, the name Georgenia muralis gen. nov., sp. nov. is proposed. The type strain is strain 1A-C(T) (=DSM 14418(T)=CCM 4963(T)). Another strain of the species is strain 3A-1 (=DSM 14419=CCM 4964).

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