International Journal of Systematic and Evolutionary Microbiology, Vol 52, 705-712, Copyright © 2002 by Society for General Microbiology

Aeromonas hydrophila subsp. dhakensis subsp. nov., isolated from children with diarrhoea in Bangladesh, and extended description of Aeromonas hydrophila subsp. hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980)

G. Huys, P. Kampfer, M. J. Albert, I. Kuhn, R. Denys and J. Swings
Laboratory of Microbiology, Ghent University, K. L. Ledeganckstr. 35, B-9000 Ghent, Belgium

A group of 10 Aeromonas hydrophila-like strains referred to as group BD-2, which mainly comprised haemolytic and cytotoxic isolates from diarrhoeal children in Bangladesh, was subjected to a polyphasic taxonomic study including fluorescent amplified fragment length polymorphism (FAFLP) and ERIC-PCR fingerprinting, microplate DNA--DNA hybridization and extensive phenotypic characterization. Comparison of FAFLP and ERIC-PCR genotypic profiles indicated that the group BD-2 isolates constituted a separate taxon that was most closely related to A. hydrophila DNA hybridization group (HG) 1. Determination of DNA--DNA hybridization values with type and reference strains of all known Aeromonas taxa revealed that the BD-2 group represented a homogeneous taxon (internal DNA homology, 77--94%) that exhibited 78--92% DNA relatedness with the type strain of A. hydrophila HG1. These results indicated that the BD-2 group belongs genotypically to A. hydrophila HG1, but examination of 152 phenotypic characteristics revealed that the group BD-2 isolates differed from representatives of HG1 in eight biochemical properties. The diagnostic value of at least three of these tests, namely utilization of urocanic acid and L-arabinose and acid production from L-arabinose, has been confirmed outside this study by other workers. Collectively, the genotypic and phenotypic data reported suggest that the BD-2 group represents a subspecies of A. hydrophila, for which the name Aeromonas hydrophila subsp. dhakensis subsp. nov. is proposed. Its type strain is P21(T) (=LMG 19562(T)=CCUG 45377(T)). An extended description of A. hydrophila subsp. hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980) is given.

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