| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
International Journal of Systematic and Evolutionary Microbiology, Vol 52, 599-605, Copyright © 2002 by Society for General Microbiology
M. Horn, T. R. Fritsche, T. Linner, R. K. Gautom, M. D. Harzenetter and M. Wagner
Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen, Am Hochanger 4, D-85350 Freising, Germany
All obligate bacterial endosymbionts of free-living amoebae currently described are affiliated with the alpha-Proteobacteria, the Chlamydiales or the phylum Cytophaga--Flavobacterium--Bacteroides. Here, six rod-shaped Gram-negative obligate bacterial endosymbionts of clinical and environmental isolates of Acanthamoeba spp. from the USA and Malaysia are reported. Comparative 16S rDNA sequence analysis demonstrated that these endosymbionts form a novel, monophyletic lineage within the beta-Proteobacteria, showing less than 90% sequence similarity to all other recognized members of this subclass. 23S rDNA sequence analysis of two symbionts confirmed this affiliation and revealed the presence of uncommon putative intervening sequences of 146 bp within helix-25 that shared no sequence homology to any other bacterial rDNA. In addition, the 23S rRNA of these endosymbionts displayed one polymorphism at the target site of oligonucleotide probe BET42a that is conserved in all other sequenced beta-Proteobacteria. Intra-cytoplasmatic localization of the endosymbionts within the amoebal host cells was confirmed by electron microscopy and fluorescence in situ hybridization with a specific 16S rRNA-targeted oligonucleotide probe. Based on these findings, the provisional name 'Candidatus Procabacter acanthamoebae' is proposed for classification of a representative of the six endosymbionts of Acanthamoeba spp. studied in this report. Comparative 18S rDNA sequence analysis of the Acanthamoeba host cells revealed their membership with either Acanthamoeba 18S rDNA sequence type T5 (Acanthamoeba lenticulata) or sequence type T4, which comprises the majority of all Acanthamoeba isolates.
This article has been cited by other articles:
|
|
 |
|
  M. Molmeret, M. Horn, M. Wagner, M. Santic, and Y. Abu Kwaik Amoebae as Training Grounds for Intracellular Bacterial Pathogens Appl. Envir. Microbiol., January 1, 2005; 71(1): 20 - 28. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Collingro, S. Poppert, E. Heinz, S. Schmitz-Esser, A. Essig, M. Schweikert, M. Wagner, and M. Horn Recovery of an environmental chlamydia strain from activated sludge by co-cultivation with Acanthamoeba sp. Microbiology, January 1, 2005; 151(1): 301 - 309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Schmitz-Esser, N. Linka, A. Collingro, C. L. Beier, H. E. Neuhaus, M. Wagner, and M. Horn ATP/ADP Translocases: a Common Feature of Obligate Intracellular Amoebal Symbionts Related to Chlamydiae and Rickettsiae J. Bacteriol., February 1, 2004; 186(3): 683 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Baker, P. Hugenholtz, S. C. Dawson, and J. F. Banfield Extremely Acidophilic Protists from Acid Mine Drainage Host Rickettsiales-Lineage Endosymbionts That Have Intervening Sequences in Their 16S rRNA Genes Appl. Envir. Microbiol., September 1, 2003; 69(9): 5512 - 5518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Beier, M. Horn, R. Michel, M. Schweikert, H.-D. Gortz, and M. Wagner The Genus Caedibacter Comprises Endosymbionts of Paramecium spp. Related to the Rickettsiales (Alphaproteobacteria) and to Francisella tularensis (Gammaproteobacteria) Appl. Envir. Microbiol., December 1, 2002; 68(12): 6043 - 6050. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
