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International Journal of Systematic and Evolutionary Microbiology, Vol 52, 457-462, Copyright © 2002 by Society for General Microbiology

Identification of isolates from soybean nodules in Xinjiang Region as Sinorhizobium xinjiangense and genetic differentiation of S. xinjiangense from Sinorhizobium fredii

G. X. Peng, Z. Y. Tan, E. T. Wang, B. Reinhold-Hurek, W. F. Chen and W. X. Chen
Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, PR China

Eight fast-growing rhizobial isolates from Xinjiang soils were identified as Sinorhizobium xinjiangense by analyses of 16S rRNA gene sequences, SDS-PAGE of proteins, intergenic spacer sequences and DNA--DNA hybridization. Based on all of the results, these isolates and the reference strains for S. xinjiangense were a distinct genomic species, although the 16S rRNA genes were closely related to that of Sinorhizobium fredii.


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