International Journal of Systematic and Evolutionary Microbiology, Vol 52, 223-228, Copyright © 2002 by Society for General Microbiology

Quadricoccus australiensis gen. nov., sp. nov., a beta-proteobacterium from activated sludge biomass

A. M. Maszenan, R. J. Seviour, BKC. Patel and P. Schumann
Biotechnology Research Centre, La Trobe University, Bendigo, VIC 3550, Australia

A Gram-negative coccus, designated strain Ben 117(T), was obtained in axenic culture by micromanipulation from an Australian activated sludge biomass sample, which had been subjected to chlorination in order to alleviate problems associated with foaming and bulking. This isolate was a strict aerobe and grew in axenic culture, also appearing in biomass samples as cocci or clusters of cocci in tetrads, thus resembling the morphotype 'G-bacteria' seen commonly in activated sludge samples. Strain Ben 117(T) was non-motile, aerobic, oxidase-negative and catalase-positive and grew between 15 and 30 degrees C, with an optimum of 25--30 degrees C. The pH range for growth was between 6.0 and 8.5, with an optimum of 7.5--8.5. The isolate stained positively for intracellular polyphosphate and poly-beta-hydroxybutyrate and its G+C content was 67 mol%. 16S rDNA sequence analysis suggests that strain Ben 117(T) is phylogenetically different from members of the genera Amaricoccus, Gram-negative 'G-bacteria' isolated previously in this laboratory. Ben 117(T) is a member of the Rhodocyclus group in the beta-Proteobacteria and equidistantly placed (similarity value of 95%) between Ferribacterium limneticum and Dechloromonas agitata (mean similarity value of 92% with the genus Rhodocyclus). Based on phenotypic and phylogenetic evidence, it is proposed that strain Ben 117(T) be designated a novel species in a new genus, Quadricoccus australiensis gen. nov., sp. nov.; the type strain is Ben 117(T) (=NCIMB 13738(T)=CIP 107055(T)).

This article has been cited by other articles:

				H.-Y. Weon, B.-Y. Kim, S.-H. Yoo, S.-W. Kwon, S.-J. Go, and E. Stackebrandt Uliginosibacterium gangwonense gen. nov., sp. nov., isolated from a wetland, Yongneup, in Korea Int J Syst Evol Microbiol, January 1, 2008; 58(1): 131 - 135. [Abstract] [Full Text] [PDF]

				B. J. Tindall and J. P. Euzeby Proposal of Parvimonas gen. nov. and Quatrionicoccus gen. nov. as replacements for the illegitimate, prokaryotic, generic names Micromonas Murdoch and Shah 2000 and Quadricoccus Maszenan et al. 2002, respectively. Int J Syst Evol Microbiol, November 1, 2006; 56(Pt 11): 2711 - 2713. [Abstract] [Full Text] [PDF]

				A. M. Maszenan, R. J. Seviour, B. K. C. Patel, P. H. Janssen, and J. Wanner Defluvicoccus vanus gen. nov., sp. nov., a novel Gram-negative coccus/coccobacillus in the 'Alphaproteobacteria' from activated sludge Int J Syst Evol Microbiol, September 1, 2005; 55(5): 2105 - 2111. [Abstract] [Full Text] [PDF]