International Journal of Systematic and Evolutionary Microbiology, Vol 52, 157-164, Copyright © 2002 by Society for General Microbiology

Anaerobaculum mobile sp. nov., a novel anaerobic, moderately thermophilic, peptide-fermenting bacterium that uses crotonate as an electron acceptor, and emended description of the genus Anaerobaculum

R. J. Menes and L. Muxi
Catedra de Microbiologia, Facultad de Quimica y Facultad de Ciencias, Universidad de la Republica, CC 1157, Montevideo, Uruguay

A novel anaerobic, moderately thermophilic, peptide-fermenting bacterium, strain NGA(T), was isolated from an anaerobic wool-scouring wastewater treatment lagoon. The cells were Gram-negative, straight rods of 0.5--1.0 x 2.0--4.0 microm, motile by means of a single flagellum. The DNA G+C content was 51.5 mol%. The optimum pH and temperature range for growth were 6.6--7.3 and 55--60 degrees C, respectively. The optimum NaCl concentration was 0.08 g l(-1). The bacterium fermented organic acids (malate, tartrate, pyruvate, glycerol and fumarate), a few carbohydrates (starch, glucose, fructose and gluconate), Casamino acids, tryptone and yeast extract. Carbohydrates and organic acids were converted to acetate, hydrogen and CO(2). The bacterium oxidized leucine to isovalerate with crotonate as an electron acceptor, but not in co-culture with Methanothermobacter thermoautotrophicus DSM 3720(T). Thiosulfate, sulfur and cystine were reduced to sulfide and crotonate was reduced to butyrate with glucose and tryptone-yeast extract as electron donors. Phylogenetic analysis of the 16S rRNA gene indicated that strain NGA(T) was related to Anaerobaculum thermoterrenum (98% similarity ), the only described species of the genus. The DNA--DNA hybridization value for strain NGA(T) and A. thermoterrenum ACM 5076(T) was 40.8%. On the basis of these results, strain NGA(T) is proposed as a novel species of the genus Anaerobaculum, namely Anaerobaculum mobile sp. nov. The type strain is NGA(T) (=DSM 13181(T)=ATCC BAA-54(T)).

This article has been cited by other articles:

				I. D. WAGNER and J. WIEGEL Diversity of Thermophilic Anaerobes Ann. N.Y. Acad. Sci., March 1, 2008; 1125(1): 1 - 43. [Abstract] [Full Text] [PDF]

				Y. Hongoh, T. Sato, M. F. Dolan, S. Noda, S. Ui, T. Kudo, and M. Ohkuma The Motility Symbiont of the Termite Gut Flagellate Caduceia versatilis Is a Member of the "Synergistes" Group Appl. Envir. Microbiol., October 1, 2007; 73(19): 6270 - 6276. [Abstract] [Full Text] [PDF]

				H. Dahle and N.-K. Birkeland Thermovirga lienii gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, amino-acid-degrading bacterium isolated from a North Sea oil well. Int J Syst Evol Microbiol, July 1, 2006; 56(Pt 7): 1539 - 1545. [Abstract] [Full Text] [PDF]

				S. Tarlera and E. B. M. Denner Sterolibacterium denitrificans gen. nov., sp. nov., a novel cholesterol-oxidizing, denitrifying member of the {beta}-Proteobacteria Int J Syst Evol Microbiol, July 1, 2003; 53(4): 1085 - 1091. [Abstract] [Full Text] [PDF]