International Journal of Systematic and Evolutionary Microbiology, Vol 51, 2049-2054, Copyright © 2001 by Society for General Microbiology

Isolation of a cinnamic acid-metabolizing Clostridium glycolicum strain from oil mill wastewaters and emendation of the species description

M Chamkha, M Labat, BKC Patel and JL Garcia
Laboratoire de Microbiologie IRD, IFR-BAIM, Universites de Provence et de la Mediterranee, ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France

A strictly anaerobic, Gram-positive, motile, sporulated bacterium, designated strain CIN5, was isolated from olive mill wastewaters after enrichment on cinnamic acid. The rod-shaped cells were slightly curved (0.4--1.1 x 2.0--15 microm) and occurred singly or in pairs. Strain CIN5 utilized a limited number of carbohydrates (glucose, fructose, maltose, sorbitol), grew optimally at 37 degrees C and at pH 7.3--7.5 and had a DNA G+C content of 29.1 +/- 0.3 mol%. Strain CIN5 was very closely related to Clostridium glycolicum DSM 1288(T). Both strain CIN5 and the type strain of C. glycolicum transformed cinnamic acid to hydrocinnamic acid and a wide range of other cinnamic acid derivatives, including o-, m- and p-coumaric, o-, m- and p-methoxycinnamic, p-methylcinnamic, caffeic, ferulic and isoferulic acids, to their corresponding 3-phenylpropionic acids by reducing the double bond of the side chain. Glucose supplementation increased the rate of conversion markedly. The emendation of the description of C. glycolicum is proposed to include these new characteristics.

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