International Journal of Systematic and Evolutionary Microbiology, Vol 51, 1881-1889, Copyright © 2001 by Society for General Microbiology

Helicobacter ganmani sp. nov., a urease-negative anaerobe isolated from the intestines of laboratory mice

BR Robertson, JL O'Rourke, P Vandamme, SLW On and A Lee
School of Microbiology and Immunology, The University of New South Wales, Sydney, NSW 2052, Australia

Spiral bacteria were isolated from the intestines of laboratory mice during a study examining the presence of Helicobacter species and other spiral organisms naturally infecting mice maintained at four different animal facilities in Sydney, Australia. One group of 17 isolates, cultured from mice from three of the four facilities, were found to be helicobacters but did not fall within any of the 18 currently recognized species. These isolates were unusual in that they only grew anaerobically at 37 degrees C and were incapable of growth under microaerobic conditions. Like Helicobacter rodentium, isolates possessed single, bipolar, unsheathed flagella and were urease-negative. They were positive for oxidase and reduced nitrate to nitrite but did not hydrolyse hippurate or indoxyl acetate, grew on charcoal agar and were resistant to cephalothin. 16S rDNA sequences from four strains were determined and found to be identical to one another. H. rodentium was the most closely related species in terms of 16S rDNA sequence similarity (98.2%). Numerical analysis of whole-cell proteins by SDS-PAGE for nine isolates was carried out with a comparison to all known Helicobacter species, including newly determined profiles from three H. rodentium strains. The new isolates were clearly differentiated from H. rodentium and other Helicobacter spp. On the basis of this data, including genetic, biochemical and protein analysis, it is proposed that these isolates belong to Helicobacter ganmani sp. nov. (type strain CMRI H02(T)=CCUG 43526(T)=CIP 106846(T)).

This article has been cited by other articles:

				T. Morner, C. Brojer, M.-P. Ryser-Degiorgis, D. Gavier-Widen, H.-O. Nilsson, and T. Wadstrom Detection of Gastric Helicobacter Species in Free-ranging Lynx (Lynx lynx) and Red Foxes (Vulpes vulpes) in Sweden J. Wildl. Dis., July 1, 2008; 44(3): 697 - 700. [Abstract] [Full Text] [PDF]

				N. S. Taylor, S. Xu, P. Nambiar, F. E. Dewhirst, and J. G. Fox Enterohepatic Helicobacter Species Are Prevalent in Mice from Commercial and Academic Institutions in Asia, Europe, and North America J. Clin. Microbiol., July 1, 2007; 45(7): 2166 - 2172. [Abstract] [Full Text] [PDF]

				H. Moyaert, A. Decostere, P. Vandamme, L. Debruyne, J. Mast, M. Baele, L. Ceelen, R. Ducatelle, and F. Haesebrouck Helicobacter equorum sp. nov., a urease-negative Helicobacter species isolated from horse faeces Int J Syst Evol Microbiol, February 1, 2007; 57(2): 213 - 218. [Abstract] [Full Text] [PDF]

				U. R. M. Bohr, M. Selgrad, C. Ochmann, S. Backert, W. Konig, A. Fenske, T. Wex, and P. Malfertheiner Prevalence and spread of enterohepatic helicobacter species in mice reared in a specific-pathogen-free animal facility. J. Clin. Microbiol., March 1, 2006; 44(3): 738 - 742. [Abstract] [Full Text] [PDF]

				B. R. Robertson, J. L. O'Rourke, B. A. Neilan, P. Vandamme, S. L. W. On, J. G. Fox, and A. Lee Mucispirillum schaedleri gen. nov., sp. nov., a spiral-shaped bacterium colonizing the mucus layer of the gastrointestinal tract of laboratory rodents Int J Syst Evol Microbiol, May 1, 2005; 55(3): 1199 - 1204. [Abstract] [Full Text] [PDF]

				M.-L. Hanninen, R. I. Karenlampi, J. M. K. Koort, T. Mikkonen, and K. J. Bjorkroth Extension of the species Helicobacter bilis to include the reference strains of Helicobacter sp. flexispira taxa 2, 3 and 8 and Finnish canine and feline flexispira strains Int J Syst Evol Microbiol, March 1, 2005; 55(2): 891 - 898. [Abstract] [Full Text] [PDF]

				A. Roers, L. Siewe, E. Strittmatter, M. Deckert, D. Schluter, W. Stenzel, A. D. Gruber, T. Krieg, K. Rajewsky, and W. Muller T Cell-specific Inactivation of the Interleukin 10 Gene in Mice Results in Enhanced T Cell Responses but Normal Innate Responses to Lipopolysaccharide or Skin Irritation J. Exp. Med., November 15, 2004; 200(10): 1289 - 1297. [Abstract] [Full Text] [PDF]

				H.-O. Nilsson, I.-S. Ouis, U. Stenram, A. Ljungh, A. P. Moran, T. Wadstrom, and W. A. Al-Soud High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR-Denaturing Gradient Gel Electrophoresis and Pyrosequencing J. Clin. Microbiol., August 1, 2004; 42(8): 3781 - 3788. [Abstract] [Full Text] [PDF]

				M.-L. Hanninen, M. Utriainen, I. Happonen, and F. E. Dewhirst Helicobacter sp. flexispira 16S rDNA taxa 1, 4 and 5 and Finnish porcine Helicobacter isolates are members of the species Helicobacter trogontum (taxon 6) Int J Syst Evol Microbiol, March 1, 2003; 53(2): 425 - 433. [Abstract] [Full Text] [PDF]

				M. Grehan, G. Tamotia, B. Robertson, and H. Mitchell Detection of Helicobacter Colonization of the Murine Lower Bowel by Genus-Specific PCR-Denaturing Gradient Gel Electrophoresis Appl. Envir. Microbiol., October 1, 2002; 68(10): 5164 - 5166. [Abstract] [Full Text] [PDF]