International Journal of Systematic and Evolutionary Microbiology, Vol 51, 1867-1871, Copyright © 2001 by Society for General Microbiology

Pigmentiphaga kullae gen. nov., sp. nov., a novel member of the family Alcaligenaceae with the ability to decolorize azo dyes aerobically

S Blumel, B Mark, HJ Busse, P Kampfer and A Stolz
Institut fur Mikrobiologie, Universitat Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany

The taxonomic position of Pseudomonas strain K24, which was isolated previously after an aerobic enrichment with the azo compound 1-(4'-carboxyphenylazo)-4-naphthol as the sole source of carbon and energy, was investigated. The detection of a quinone system with ubiquinone Q-8 as the predominant compound and a polyamine pattern with putrescine and 2-hydroxyputrescine as the major polyamines present suggested that strain K24(T) belongs to the -subclass of the Proteobacteria. This was supported by sequencing the 16S rRNA gene, which demonstrated about 95--96% sequence similarity to different species of the genera Achromobacter, Alcaligenes and Bordetella. This suggested that strain K24(T) is a member of the family Alcaligenaceae. The G+C content of the DNA was 68.5 mol%. Different methods for the construction of phylogenetic dendrograms placed strain K24(T) separate from the genera Alcaligenes, Achromobacter and Bordetella. Analysis of the fatty acids demonstrated the presence of 10:0 3-OH and high concentrations of summed feature 7 (18:1omega7c, 18:1omega9t and/or 18:1omega12t) and 19:0 cyclo-omega8c, which is unique among previously described species of the genera Alcaligenes, Achromobacter and Bordetella. On the basis of the low 16S rRNA sequence similarities, the composition of the fatty acid profile and unique phenotypic properties, a new genus and species is proposed for strain K24(T) with the name Pigmentiphaga kullae gen. nov., sp. nov.

This article has been cited by other articles:

				J.-H. Yoon, S.-J. Kang, W. Kim, and T.-K. Oh Pigmentiphaga daeguensis sp. nov., isolated from wastewater of a dye works, and emended description of the genus Pigmentiphaga Int J Syst Evol Microbiol, June 1, 2007; 57(6): 1188 - 1191. [Abstract] [Full Text] [PDF]

				P. Kampfer, K. Denger, A. M. Cook, S.-T. Lee, U. Jackel, E. B. M. Denner, and H.-J. Busse Castellaniella gen. nov., to accommodate the phylogenetic lineage of Alcaligenes defragrans, and proposal of Castellaniella defragrans gen. nov., comb. nov. and Castellaniella denitrificans sp. nov. Int J Syst Evol Microbiol, April 1, 2006; 56(Pt 4): 815 - 819. [Abstract] [Full Text] [PDF]

				W. Ghosh, A. Bagchi, S. Mandal, B. Dam, and P. Roy Tetrathiobacter kashmirensis gen. nov., sp. nov., a novel mesophilic, neutrophilic, tetrathionate-oxidizing, facultatively chemolithotrophic betaproteobacterium isolated from soil from a temperate orchard in Jammu and Kashmir, India Int J Syst Evol Microbiol, September 1, 2005; 55(5): 1779 - 1787. [Abstract] [Full Text] [PDF]

				A. Stolz, S. Burger, A. Kuhm, P. Kampfer, and H.-J. Busse Pusillimonas noertemannii gen. nov., sp. nov., a new member of the family Alcaligenaceae that degrades substituted salicylates Int J Syst Evol Microbiol, May 1, 2005; 55(3): 1077 - 1081. [Abstract] [Full Text] [PDF]

				T. Coenye, E. Vanlaere, E. Samyn, E. Falsen, P. Larsson, and P. Vandamme Advenella incenata gen. nov., sp. nov., a novel member of the Alcaligenaceae, isolated from various clinical samples Int J Syst Evol Microbiol, January 1, 2005; 55(1): 251 - 256. [Abstract] [Full Text] [PDF]

				T. Coenye, M. Vancanneyt, M. C. Cnockaert, E. Falsen, J. Swings, and P. Vandamme Kerstersia gyiorum gen. nov., sp. nov., a novel Alcaligenes faecalis-like organism isolated from human clinical samples, and reclassification of Alcaligenes denitrificans Ruger and Tan 1983 as Achromobacter denitrificans comb. nov. Int J Syst Evol Microbiol, November 1, 2003; 53(6): 1825 - 1831. [Abstract] [Full Text] [PDF]

				S. Tarlera and E. B. M. Denner Sterolibacterium denitrificans gen. nov., sp. nov., a novel cholesterol-oxidizing, denitrifying member of the {beta}-Proteobacteria Int J Syst Evol Microbiol, July 1, 2003; 53(4): 1085 - 1091. [Abstract] [Full Text] [PDF]

				S. Blumel, H.-J. Knackmuss, and A. Stolz Molecular Cloning and Characterization of the Gene Coding for the Aerobic Azoreductase from Xenophilus azovorans KF46F Appl. Envir. Microbiol., August 1, 2002; 68(8): 3948 - 3955. [Abstract] [Full Text] [PDF]