International Journal of Systematic and Evolutionary Microbiology, Vol 51, 1539-1548, Copyright © 2001 by Society for General Microbiology

Thermoanaerobacter yonseiensis sp. nov., a novel extremely thermophilic, xylose-utilizing bacterium that grows at up to 85 degrees C

BC Kim, R Grote, DW Lee, G Antranikian and YR Pyun
Department of Biotechnology and Bioproducts Research Center, Yonsei University, Seodaemungu Shinchondong 134, Seoul 120-749, Korea

A novel strictly anaerobic, extremely thermophilic, spore-forming and xylose-utilizing bacterium, designated strain KB-1(TP) (type and patent strain), was isolated from a geothermal hot stream at Sileri on Java island, Indonesia. The cells were rod-shaped, motile and had terminal spores. The newly isolated strain stained Gram-positive and the cells occurred singly or in pairs during the exponential growth phase. The temperature optimum for growth was 75 degrees C and growth occurred in the range 50--85 degrees C. The pH range for growth was 4.5--9.0, with an optimum at pH 6.5. Strain KB-1(TP) grew chemo-organotrophically by fermenting a wide range of substrates such as glucose, fructose, D-xylose, lactose, maltose, sucrose, mannose, galactose, cellobiose, pullulan and soluble starch. Arabinose, xylan, cellulose, olive oil and Tween 80 were not fermented. The predominant fermentation end products after growth on glucose were lactate, acetate, ethanol, CO(2) and small amounts of isovaleric acid, butyric acid, propionic acid, 1-pentanol and 2-propanol. Thiosulfate was reduced to H(2)S. Strain KB-1(TP) was sensitive to tetracycline, chloramphenicol, penicillin G, neomycin, kanamycin, vancomycin and rifampicin at concentrations of 100 microg ml(-1). No effect was observed with chloramphenicol and neomycin at concentrations of 10 microg ml(-1). This indicates that strain KB-1(TP) belongs to the bacterial domain. The G+C content of the DNA was 37 mol%. The comparison of the 16S rDNA sequence to that of closely related strains revealed that strain KB-1(TP) belonged to clostridial cluster V, showing highest sequence identities (92.7%) to members of the genus Thermoanaerobacter. Taking into account the physiological and molecular properties of the new isolate, it is proposed that strain KB-1(TP) should be classified as a new species of the genus Thermoanaerobacter, designated Thermoanaerobacter yonseiensis. The type strain, KB-1(TP), has been deposited in the Korean Federation of Culture Collections (KFCC 11116(P)) as a patent strain and in the Deutsche Sammlung von Mikroorganismen und Zellkulturen as a type strain (=DSM 13777(T)).

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