International Journal of Systematic and Evolutionary Microbiology, Vol 51, 999-1006, Copyright © 2001 by Society for General Microbiology

Comamonas denitrificans sp. nov., an efficient denitrifying bacterium isolated from activated sludge

L Gumaelius, G Magnusson, B Pettersson and G Dalhammar
Department of Biotechnology, Royal Institute of Technology, KTH, S-100 44 Stockholm, Sweden

To find a biomarker for denitrification in activated sludge, five denitrifying strains isolated from three wastewater treatment plants were studied. These strains were selected from among 1500 isolates for their excellent denitrifying properties. They denitrify quickly and have no lag phase when switching from aerobic to anoxic conditions. All strains have the cd(1)-type of nitrite reductase. The strains are Gram-negative rods and they all grow as filamentous chains when cultivated in liquid solution. The strains differ in colony morphology when grown on nutrient agar. Almost full-length 16S rDNA sequences were determined and phylogenetic analysis revealed that these strains are positioned among members of the genus Comamonas in the beta-subclass of the Proteobacteria. Signature nucleotides and bootstrap percentages were also analysed to verify this position. Strains 110, 123(T), 2.99g, 5.38g and P17 were </=96.7% similar to known strains, but >/=99.7% similar to each other, as judged from their 16S rDNA sequences, and grouped tightly together in the phylogenetic tree. Sequence motifs in the 16S rRNA gene were also found, suggesting the monophyletic origin of these strains. Nevertheless, some strains differed from the others, for example strain 110 branches early from the other strains and 5.38g is phenotypically more inert. Therefore, it is proposed that strains 110, 123(T), 2.99g and P17 are classified into a new species, Comamonas denitrificans sp. nov., while the taxonomic status of strain 5.38g will have to await the outcome of further studies. The type strain of Comamonas denitrificans is 123(T) (ATCC 700936(T)).

This article has been cited by other articles:

				K. Heylen, L. Lebbe, and P. De Vos Acidovorax caeni sp. nov., a denitrifying species with genetically diverse isolates from activated sludge Int J Syst Evol Microbiol, January 1, 2008; 58(1): 73 - 77. [Abstract] [Full Text] [PDF]

				C.-C. Young, J.-H. Chou, A. B. Arun, W.-S. Yen, S.-Y. Sheu, F.-T. Shen, W.-A. Lai, P. D. Rekha, and W.-M. Chen Comamonas composti sp. nov., isolated from food waste compost Int J Syst Evol Microbiol, January 1, 2008; 58(1): 251 - 256. [Abstract] [Full Text] [PDF]

				A. Oceguera-Cervantes, A. Carrillo-Garcia, N. Lopez, S. Bolanos-Nunez, M. J. Cruz-Gomez, C. Wacher, and H. Loza-Tavera Characterization of the Polyurethanolytic Activity of Two Alicycliphilus sp. Strains Able To Degrade Polyurethane and N-Methylpyrrolidone Appl. Envir. Microbiol., October 1, 2007; 73(19): 6214 - 6223. [Abstract] [Full Text] [PDF]

				J.-H. Chou, S.-Y. Sheu, K.-Y. Lin, W.-M. Chen, A. B. Arun, and C.-C. Young Comamonas odontotermitis sp. nov., isolated from the gut of the termite Odontotermes formosanus Int J Syst Evol Microbiol, April 1, 2007; 57(4): 887 - 891. [Abstract] [Full Text] [PDF]

				E. Cytryn, J. van Rijn, A. Schramm, A. Gieseke, D. de Beer, and D. Minz Identification of Bacteria Potentially Responsible for Oxic and Anoxic Sulfide Oxidation in Biofilters of a Recirculating Mariculture System Appl. Envir. Microbiol., October 1, 2005; 71(10): 6134 - 6141. [Abstract] [Full Text] [PDF]

				C.-P. Yu, R. Ahuja, G. Sayler, and K.-H. Chu Quantitative Molecular Assay for Fingerprinting Microbial Communities of Wastewater and Estrogen-Degrading Consortia Appl. Envir. Microbiol., March 1, 2005; 71(3): 1433 - 1444. [Abstract] [Full Text] [PDF]

				L. M. Junker and A. G. Hay Effects of triclosan incorporation into ABS plastic on biofilm communities J. Antimicrob. Chemother., June 1, 2004; 53(6): 989 - 996. [Abstract] [Full Text] [PDF]

				S. Spring, U. Jackel, M. Wagner, and P. Kampfer Ottowia thiooxydans gen. nov., sp. nov., a novel facultatively anaerobic, N2O-producing bacterium isolated from activated sludge, and transfer of Aquaspirillum gracile to Hylemonella gracilis gen. nov., comb. nov. Int J Syst Evol Microbiol, January 1, 2004; 54(1): 99 - 106. [Abstract] [Full Text] [PDF]

				G. Wauters, T. De Baere, A. Willems, E. Falsen, and M. Vaneechoutte Description of Comamonas aquatica comb. nov. and Comamonas kerstersii sp. nov. for two subgroups of Comamonas terrigena and emended description of Comamonas terrigena Int J Syst Evol Microbiol, May 1, 2003; 53(3): 859 - 862. [Abstract] [Full Text] [PDF]