International Journal of Systematic and Evolutionary Microbiology, Vol 51, 933-936, Copyright © 2001 by Society for General Microbiology

Nocardia veterana sp. nov., isolated from human bronchial lavage

V Gurtler, R Smith, BC Mayall, G Potter-Reinemann, E Stackebrandt and RM Kroppenstedt
Department of Microbiology, Austin & Repatriation Medical Center (Austin Campus), Studley Road, Heidelberg, Vic. 3084, Australia

A nocardioform bacterium was isolated from the bronchoscopic lavage of a 78-year-old man with a past history of tuberculous pleurisy, who presented with bilateral upper lobe lesions at Austin and Repatriation Medical Centre, Heidelberg, Australia. The strain was aerobic, Gram-positive, produced beige substrate mycelium and scant white aerial mycelium. It showed chemotaxonomic markers which were consistent with the classification of Nocardia: i.e. meso-diaminopimelic acid, N-glycolylmuramic acid, arabinose and galactose as diagnostic sugars; phospholipids phosphatidylinositol mannosides, phosphatidylinositol, phosphatidylethanolamine and diphosphatidylglycerol; a menaquinone with a cyclic isoprene side chain, MK-8(H(4cycl.)); a fatty acid pattern composed of unbranched saturated and monounsaturated fatty acids with a considerable amount of tuberculostearic acid; and mycolic acids composed of 54--62 carbon atoms with three principal mycolic acids which were mono- and polyunsaturated, showing a chain length C(56), C(58) and C(60) and accounting for over 70% of the entire pattern. The 16S rDNA sequence showed the highest similarity to the type strain of Nocardia vaccinii; the DNA--DNA similarity of the two strains was 31%. These data, together with distinct physiological traits and molecular biological analyses, as well as chemotaxonomic results, led to the conclusion that the novel isolate represents a new species within the genus Nocardia for which the name Nocardia veterana sp. nov. is proposed. The type strain is M157222(T) (DSM 44445(T)=NRRL B-24136(T)).

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