International Journal of Systematic and Evolutionary Microbiology, Vol 51, 843-852, Copyright © 2001 by Society for General Microbiology

Corynebacterium mooreparkense sp. nov. and Corynebacterium casei sp. nov., isolated from the surface of a smear-ripened cheese

NM Brennan, R Brown, M Goodfellow, AC Ward, TP Beresford, PJ Simpson, PF Fox and TM Cogan
Dairy Products Research Centre, Teagasc, Moorepark, Fermoy, County Cork, Ireland

Ten isolates each of two different bacterial species isolated from the surface of a smear-ripened cheese were found to exhibit many characteristics of the genus Corynebacterium. The isolates were Gram-positive, catalase-positive, non-spore-forming rods that did not undergo a rod/coccus transformation when grown on complex media. Chemotaxonomic investigation revealed that the strains belonged unambiguously to the genus Corynebacterium. Their cell walls contained arabinose, galactose and short-chain mycolic acids (C(22) to C(36)) and their peptidoglycan contained meso-diaminopimelic acid. The G+C content of the DNA was 51--60 mol%. MK-9 (H(2)) was the principal menaquinone. The 16S rDNA sequences of four isolates of each bacterium were determined and aligned with those of other members of the coryneform group. Phylogenetic analysis showed that the strains represented two new sublines within the genus Corynebacterium; Corynebacterium variabile and Corynebacterium ammoniagenes were their nearest known phylogenetic neighbours. Corynebacterium variabile and Corynebacterium ammoniagenes showed the highest levels of sequence homology with the isolates; however, DNA--DNA hydridization studies indicated that the Corynebacterium strains isolated from the cheese smear did not belong to either Corynebacterium variabile or Corynebacterium ammoniagenes (26 and 46% chromosomal similarity, respectively). On the basis of the phylogenetic and phenotypic distinctiveness of the unknown isolates, it is proposed that the bacteria be classified as two new Corynebacterium species, for which the names Corynebacterium mooreparkense sp. nov. and Corynebacterium casei sp. nov. are proposed. Type strains have been deposited in culture collections as Corynebacterium mooreparkense LMG S-19265(T) (=NCIMB 30131(T)) and Corynebacterium casei LMG S-19264(T) (=NCIMB 30130(T)).

This article has been cited by other articles:

				J. Mounier, M. C. Rea, P. M. O'Connor, G. F. Fitzgerald, and T. M. Cogan Growth Characteristics of Brevibacterium, Corynebacterium, Microbacterium, and Staphylococcus spp. Isolated from Surface-Ripened Cheese Appl. Envir. Microbiol., December 1, 2007; 73(23): 7732 - 7739. [Abstract] [Full Text] [PDF]

				M. Ventura, C. Canchaya, A. Tauch, G. Chandra, G. F. Fitzgerald, K. F. Chater, and D. van Sinderen Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum Microbiol. Mol. Biol. Rev., September 1, 2007; 71(3): 495 - 548. [Abstract] [Full Text] [PDF]

				M. C. Rea, S. Gorges, R. Gelsomino, N. M. Brennan, J. Mounier, M. Vancanneyt, S. Scherer, J. Swings, and T. M. Cogan Stability of the Biodiversity of the Surface Consortia of Gubbeen, a Red-Smear Cheese J Dairy Sci, May 1, 2007; 90(5): 2200 - 2210. [Abstract] [Full Text] [PDF]

				N. Bora, M. Vancanneyt, R. Gelsomino, J. Swings, N. Brennan, T. M. Cogan, S. Larpin, N. Desmasures, F. E. Lechner, R. M. Kroppenstedt, et al. Agrococcus casei sp. nov., isolated from the surfaces of smear-ripened cheeses Int J Syst Evol Microbiol, January 1, 2007; 57(1): 92 - 97. [Abstract] [Full Text] [PDF]

				C. Monnet, K. Correia, A.-S. Sarthou, and F. Irlinger Quantitative Detection of Corynebacterium casei in Cheese by Real-Time PCR Appl. Envir. Microbiol., November 1, 2006; 72(11): 6972 - 6979. [Abstract] [Full Text] [PDF]

				X.-H. Shen, C.-Y. Jiang, Y. Huang, Z.-P. Liu, and S.-J. Liu Functional Identification of Novel Genes Involved in the Glutathione-Independent Gentisate Pathway in Corynebacterium glutamicum Appl. Envir. Microbiol., July 1, 2005; 71(7): 3442 - 3452. [Abstract] [Full Text] [PDF]

				R. Gelsomino, M. Vancanneyt, C. Snauwaert, K. Vandemeulebroecke, B. Hoste, T. M. Cogan, and J. Swings Corynebacterium mooreparkense, a later heterotypic synonym of Corynebacterium variabile Int J Syst Evol Microbiol, May 1, 2005; 55(3): 1129 - 1131. [Abstract] [Full Text] [PDF]

				F. Irlinger, F. Bimet, J. Delettre, M. Lefevre, and P. A. D. Grimont Arthrobacter bergerei sp. nov. and Arthrobacter arilaitensis sp. nov., novel coryneform species isolated from the surfaces of cheeses Int J Syst Evol Microbiol, January 1, 2005; 55(1): 457 - 462. [Abstract] [Full Text] [PDF]

				C. Feurer, D. Clermont, F. Bimet, A. Candrea, M. Jackson, P. Glaser, C. Bizet, and C. Dauga Taxonomic characterization of nine strains isolated from clinical and environmental specimens, and proposal of Corynebacterium tuberculostearicum sp. nov. Int J Syst Evol Microbiol, July 1, 2004; 54(4): 1055 - 1061. [Abstract] [Full Text] [PDF]

				A. I. Vela, A. Mateos, M. D. Collins, V. Briones, R. A. Hutson, L. Dominguez, and J. F. Fernandez-Garayzabal Corynebacterium suicordis sp. nov., from pigs Int J Syst Evol Microbiol, November 1, 2003; 53(6): 2027 - 2031. [Abstract] [Full Text] [PDF]

				A. Maoz, R. Mayr, and S. Scherer Temporal Stability and Biodiversity of Two Complex Antilisterial Cheese-Ripening Microbial Consortia Appl. Envir. Microbiol., July 1, 2003; 69(7): 4012 - 4018. [Abstract] [Full Text] [PDF]

				A. F. Yassin, R. M. Kroppenstedt, and W. Ludwig Corynebacterium glaucum sp. nov. Int J Syst Evol Microbiol, May 1, 2003; 53(3): 705 - 709. [Abstract] [Full Text] [PDF]

				N. M. Brennan, A. C. Ward, T. P. Beresford, P. F. Fox, M. Goodfellow, and T. M. Cogan Biodiversity of the Bacterial Flora on the Surface of a Smear Cheese Appl. Envir. Microbiol., February 1, 2002; 68(2): 820 - 830. [Abstract] [Full Text] [PDF]

				G. E. Gardiner, C. Heinemann, A. W. Bruce, D. Beuerman, and G. Reid Persistence of Lactobacillus fermentum RC-14 and Lactobacillus rhamnosus GR-1 but Not L. rhamnosus GG in the Human Vagina as Demonstrated by Randomly Amplified Polymorphic DNA Clin. Vaccine Immunol., January 1, 2002; 9(1): 92 - 96. [Abstract] [Full Text] [PDF]