| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
International Journal of Systematic and Evolutionary Microbiology, Vol 51, 505-512, Copyright © 2001 by Society for General Microbiology
MC Moffitt, SI Blackburn and BA Neilan
School of Microbiology and Immunology, The University of New South Wales, Sydney, NSW 2052, Australia
Nodularia, a member of the order Nostocales, is a bloom-forming filamentous cyanobacterium that possesses the ability to form toxic blooms. The toxin produced by Nodularia, nodularin, is a hepatotoxin, similar in structure to the heptapeptide toxin microcystin. Twenty-one strains of Nodularia, representing the species Nodularia spumigena, Nodularia harveyana and Nodularia sphaerocarpa, were analysed for toxin production by protein phosphatase inhibition assay and sequenced over the 16S rDNA region. Phylogenetic analysis of Nodularia 16S rDNA sequences found that Nodularia clustered into two main groups. An N. spumigena cluster was distinct from the benthic species N. harveyana and N. sphaerocarpa. There was no distinction between strains isolated from globally diverse locations. Nodularin-producing species were restricted to the single, evolutionally distinct cluster of N. spumigena. This observation has enabled the design of a specific 16S rRNA PCR for the rapid detection of nodularin-producing strains. Alignment of 16S rDNA sequences from toxic and non-toxic Nodularia with other members of the cyanobacteria allowed the design of both Nodularia generic and toxic N. spumigena-specific primers.
This article has been cited by other articles:
|
|
 |
|
  E. Gorokhova and J. Engstrom-Ost Toxin concentration in Nodularia spumigena is modulated by mesozooplankton grazers J. Plankton Res., October 1, 2009; 31(10): 1235 - 1247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Palinska, C. F. Thomasius, J. Marquardt, and S. Golubic Phylogenetic evaluation of cyanobacteria preserved as historic herbarium exsiccata. Int J Syst Evol Microbiol, October 1, 2006; 56(Pt 10): 2253 - 2263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lyra, M. Laamanen, J. M. Lehtimaki, A. Surakka, and K. Sivonen Benthic cyanobacteria of the genus Nodularia are non-toxic, without gas vacuoles, able to glide and genetically more diverse than planktonic Nodularia Int J Syst Evol Microbiol, March 1, 2005; 55(2): 555 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Moffitt and B. A. Neilan Characterization of the Nodularin Synthetase Gene Cluster and Proposed Theory of the Evolution of Cyanobacterial Hepatotoxins Appl. Envir. Microbiol., November 1, 2004; 70(11): 6353 - 6362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Baker, B. Entsch, B. A. Neilan, and D. B. McKay Monitoring Changing Toxigenicity of a Cyanobacterial Bloom by Molecular Methods Appl. Envir. Microbiol., December 1, 2002; 68(12): 6070 - 6076. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Iteman, R. Rippka, N. Tandeau de Marsac, and M. Herdman rDNA analyses of planktonic heterocystous cyanobacteria, including members of the genera Anabaenopsis and Cyanospira Microbiology, February 1, 2002; 148(2): 481 - 496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Laamanen, M. F. Gugger, J. M. Lehtimaki, K. Haukka, and K. Sivonen Diversity of Toxic and Nontoxic Nodularia Isolates (Cyanobacteria) and Filaments from the Baltic Sea Appl. Envir. Microbiol., October 1, 2001; 67(10): 4638 - 4647. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
