| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
International Journal of Systematic and Evolutionary Microbiology, Vol 51, 271-279, Copyright © 2001 by Society for General Microbiology
T Coenye, JJ LiPuma, D Henry, B Hoste, K Vandemeulebroecke, M Gillis, DP Speert and P Vandamme
Laboratorium voor Microbiologie, Universiteit Gent, K.-L. Ledeganckstraat 35, Ghent, Belgium
A polyphasic taxonomic study was performed on 23 strains isolated from cystic fibrosis (CF) patients in the USA. These strains were tentatively identified as Burkholderia cepacia, Burkholderia vietnamiensis and Burkholderia or Ralstonia sp. using biochemical tests and 16S rDNA-based PCR assays. Visual comparison of protein profiles indicated that they belonged to a single new group ('group 13'). The polyphasic taxonomic data showed that 18 of these strains represent a new member of the B. cepacia complex, referred to in this report as B. cepacia genomovar VI, whereas the other five strains belonged to Burkholderia multivorans. By means of biochemical tests, B. cepacia genomovar VI strains can be separated from B. cepacia genomovars I and III, Burkholderia stabilis, B. vietnamiensis and Burkholderia gladioli, but not from B. multivorans. Separation of B. cepacia genomovar VI and B. multivorans is possible using AFLP (amplified fragment length polymorphism) fingerprinting and DNA--DNA hybridizations. Retrospective analysis of epidemiological and genotypic data suggests that strains of B. cepacia genomovar VI have been involved in chronic colonization of CF patients and have been spread from person to person.
This article has been cited by other articles:
|
|
 |
|
  R. J. Malott, E. P. O'Grady, J. Toller, S. Inhulsen, L. Eberl, and P. A. Sokol A Burkholderia cenocepacia Orphan LuxR Homolog Is Involved in Quorum-Sensing Regulation J. Bacteriol., April 15, 2009; 191(8): 2447 - 2460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Jacobs, A. C. Fasi, A. Ramette, J. J. Smith, R. Hammerschmidt, and G. W. Sundin Identification and Onion Pathogenicity of Burkholderia cepacia Complex Isolates from the Onion Rhizosphere and Onion Field Soil Appl. Envir. Microbiol., May 15, 2008; 74(10): 3121 - 3129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Seed and J. J. Dennis Development of Galleria mellonella as an Alternative Infection Model for the Burkholderia cepacia Complex Infect. Immun., March 1, 2008; 76(3): 1267 - 1275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. P. Partida-Martinez, I. Groth, I. Schmitt, W. Richter, M. Roth, and C. Hertweck Burkholderia rhizoxinica sp. nov. and Burkholderia endofungorum sp. nov., bacterial endosymbionts of the plant-pathogenic fungus Rhizopus microsporus Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2583 - 2590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Keto-Timonen, A. Heikinheimo, E. Eerola, and H. Korkeala Identification of Clostridium Species and DNA Fingerprinting of Clostridium perfringens by Amplified Fragment Length Polymorphism Analysis J. Clin. Microbiol., November 1, 2006; 44(11): 4057 - 4065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Perin, L. Martinez-Aguilar, G. Paredes-Valdez, J. I. Baldani, P. Estrada-de los Santos, V. M. Reis, and J. Caballero-Mellado Burkholderia silvatlantica sp. nov., a diazotrophic bacterium associated with sugar cane and maize. Int J Syst Evol Microbiol, August 1, 2006; 56(Pt 8): 1931 - 1937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.-P. Vonberg, S. Haussler, P. Vandamme, and I. Steinmetz Identification of Burkholderia cepacia complex pathogens by rapid-cycle PCR with fluorescent hybridization probes J. Med. Microbiol., June 1, 2006; 55(6): 721 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Kalish, D. A. Waltz, M. Dovey, G. Potter-Bynoe, A. J. McAdam, J. J. LiPuma, C. Gerard, and D. Goldmann Impact of Burkholderia dolosa on Lung Function and Survival in Cystic Fibrosis Am. J. Respir. Crit. Care Med., February 15, 2006; 173(4): 421 - 425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gingues, C. Kooi, M. B. Visser, B. Subsin, and P. A. Sokol Distribution and Expression of the ZmpA Metalloprotease in the Burkholderia cepacia Complex J. Bacteriol., December 15, 2005; 187(24): 8247 - 8255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. Kim, U. S. Sajjan, G. P. Krasan, and J. J. LiPuma Disruption of Tight Junctions during Traversal of the Respiratory Epithelium by Burkholderia cenocepacia Infect. Immun., November 1, 2005; 73(11): 7107 - 7112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Mahenthiralingam and P Vandamme Taxonomy and pathogenesis of the Burkholderia cepacia complex Chronic Respiratory Disease, October 1, 2005; 2(4): 209 - 217. [Abstract] [PDF]
|
|
|
|
|
|
K. L. Tomlin, R. J. Malott, G. Ramage, D. G. Storey, P. A. Sokol, and H. Ceri Quorum-Sensing Mutations Affect Attachment and Stability of Burkholderia cenocepacia Biofilms Appl. Envir. Microbiol., September 1, 2005; 71(9): 5208 - 5218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Reik, T. Spilker, and J. J. LiPuma Distribution of Burkholderia cepacia Complex Species among Isolates Recovered from Persons with or without Cystic Fibrosis J. Clin. Microbiol., June 1, 2005; 43(6): 2926 - 2928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Cheng and B. J. Currie Melioidosis: Epidemiology, Pathophysiology, and Management Clin. Microbiol. Rev., April 1, 2005; 18(2): 383 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ramette, J. J. LiPuma, and J. M. Tiedje Species Abundance and Diversity of Burkholderia cepacia Complex in the Environment Appl. Envir. Microbiol., March 1, 2005; 71(3): 1193 - 1201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. De Soyza, C. D. Ellis, C. M. A. Khan, P. A. Corris, and R. D. de Hormaeche Burkholderia cenocepacia Lipopolysaccharide, Lipid A, and Proinflammatory Activity Am. J. Respir. Crit. Care Med., July 1, 2004; 170(1): 70 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. De Boeck, A. Malfroot, L. Van Schil, P. Lebecque, C. Knoop, J.R.W. Govan, C. Doherty, S. Laevens, and P. Vandamme Epidemiology of Burkholderia cepacia complex colonisation in cystic fibrosis patients Eur. Respir. J., June 1, 2004; 23(6): 851 - 856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Vermis, T. Coenye, J. J. LiPuma, E. Mahenthiralingam, H. J. Nelis, and P. Vandamme Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov. Int J Syst Evol Microbiol, May 1, 2004; 54(3): 689 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Bernier, L. Silo-Suh, D. E. Woods, D. E. Ohman, and P. A. Sokol Comparative Analysis of Plant and Animal Models for Characterization of Burkholderia cepacia Virulence Infect. Immun., September 1, 2003; 71(9): 5306 - 5313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Cunha, J. H. Leitao, E. Mahenthiralingam, P. Vandamme, L. Lito, C. Barreto, M. J. Salgado, and I. Sa-Correia Molecular Analysis of Burkholderia cepacia Complex Isolates from a Portuguese Cystic Fibrosis Center: a 7-Year Study J. Clin. Microbiol., September 1, 2003; 41(9): 4113 - 4120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Detsika, J. E. Corkill, M. Magalhaes, K. J. Glendinning, C. A. Hart, and C. Winstanley Molecular Typing of, and Distribution of Genetic Markers among, Burkholderia cepacia Complex Isolates from Brazil J. Clin. Microbiol., September 1, 2003; 41(9): 4148 - 4153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Vinion-Dubiel and J. B. Goldberg Review: Lipopolysaccharide of Burkholderia cepacia complex Innate Immunity, August 1, 2003; 9(4): 201 - 213. [Abstract] [PDF]
|
|
|
|
|
|
T. Coenye, J. Goris, P. De Vos, P. Vandamme, and J. J. LiPuma Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov. Int J Syst Evol Microbiol, July 1, 2003; 53(4): 1075 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ramisse, J. Balandreau, F. Thibault, D. Vidal, G. Vergnaud, and P. Normand DNA-DNA hybridization study of Burkholderia species using genomic DNA macro-array analysis coupled to reverse genome probing Int J Syst Evol Microbiol, May 1, 2003; 53(3): 739 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tomich, A. Griffith, C. A. Herfst, J. L. Burns, and C. D. Mohr Attenuated Virulence of a Burkholderia cepacia Type III Secretion Mutant in a Murine Model of Infection Infect. Immun., March 1, 2003; 71(3): 1405 - 1415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. VERMIS, T. COENYE, E. MAHENTHIRALINGAM, H. J. NELIS, and P. VANDAMME Evaluation of species-specific recA-based PCR tests for genomovar level identification within the Burkholderia cepacia complex J. Med. Microbiol., November 1, 2002; 51(11): 937 - 940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Brisse, S. Stefani, J. Verhoef, A. Van Belkum, P. Vandamme, and W. Goessens Comparative Evaluation of the BD Phoenix and VITEK 2 Automated Instruments for Identification of Isolates of the Burkholderia cepacia Complex J. Clin. Microbiol., May 1, 2002; 40(5): 1743 - 1748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Salles, F. A. De Souza, and J. D. van Elsas Molecular Method To Assess the Diversity of Burkholderia Species in Environmental Samples Appl. Envir. Microbiol., April 1, 2002; 68(4): 1595 - 1603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tomich, C. A. Herfst, J. W. Golden, and C. D. Mohr Role of Flagella in Host Cell Invasion by Burkholderia cepacia Infect. Immun., April 1, 2002; 70(4): 1799 - 1806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bevivino, C. Dalmastri, S. Tabacchioni, L. Chiarini, M. L. Belli, S. Piana, A. Materazzo, P. Vandamme, and G. Manno Burkholderia cepacia Complex Bacteria from Clinical and Environmental Sources in Italy: Genomovar Status and Distribution of Traits Related to Virulence and Transmissibility J. Clin. Microbiol., March 1, 2002; 40(3): 846 - 851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. McDowell, E. Mahenthiralingam, J. E. Moore, K. E. A. Dunbar, A. K. Webb, M. E. Dodd, S. L. Martin, B. C. Millar, C. J. Scott, M. Crowe, et al. PCR-Based Detection and Identification of Burkholderiacepacia Complex Pathogens in Sputum from Cystic Fibrosis Patients J. Clin. Microbiol., December 1, 2001; 39(12): 4247 - 4255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Coenye, P. Vandamme, J. R. W. Govan, and J. J. LiPuma Taxonomy and Identification of the Burkholderia cepacia Complex J. Clin. Microbiol., October 1, 2001; 39(10): 3427 - 3436. [Full Text] [PDF]
|
|
|
|
|
|
A. Agodi, E. Mahenthiralingam, M. Barchitta, V. Giannino, A. Sciacca, and S. Stefani Burkholderia cepacia Complex Infection in Italian Patients with Cystic Fibrosis: Prevalence, Epidemiology, and Genomovar Status J. Clin. Microbiol., August 1, 2001; 39(8): 2891 - 2896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lutter, S. Lewenza, J. J. Dennis, M. B. Visser, and P. A. Sokol Distribution of Quorum-Sensing Genes in the Burkholderia cepacia Complex Infect. Immun., July 1, 2001; 69(7): 4661 - 4666. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
