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International Journal of Systematic and Evolutionary Microbiology, Vol 50, 1909-1913, Copyright © 2000 by Society for General Microbiology
J Chun, KS Bae, EY Moon, SO Jung, HK Lee and SJ Kim
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusong, Taejon 305-600, Republic of Korea
A moderately halophilic actinomycete, designated HA-9(T), was isolated from a saltern in Kunsan, Republic of Korea, and was the subject of polyphasic identification. Analysis of 16S rDNA indicated that the isolate belonged to the genus Nocardiopsis, but differed genetically from other Nocardiopsis species. Strain HA-9(T) contained meso-diaminopimelic acid, no diagnostic sugars, hexa- or octa-hydrogenated menaquinones with 10 isoprene units, straight-chain saturated or monounsaturated, iso-, anteiso-, 10-methyl branched fatty acids with 13--18 carbons and type III phospholipids. All of these characters consistently assign the isolate to the genus Nocardiopsis. All of the validly described Nocardiopsis species, including moderately halophilic Nocardiopsis halophila, can be differentiated from the saltern isolate using morphological and physiological traits. On the basis of polyphasic evidence, the name Nocardiopsis kunsanensis sp. nov. is proposed for strain HA-9(T) (=KCTC 9831(T)), which is designated the type strain.
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