| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
International Journal of Systematic and Evolutionary Microbiology, Vol 50, 1629-1639, Copyright © 2000 by Society for General Microbiology
C Guasp, ERB Moore, J Lalucat and A Bennasar
Microbiologia, Departament de Biologia, Institut Mediterrani d'Estudis Avancats (CSIC-UIB), Palma de Mallorca, Spain
Bacteria identified and classified as Pseudomonas stutzeri, on the basis of traditional criteria, are recognized to be markedly heterogeneous, such that a systematic phenotypic characterization has not been correlated with genotypic groupings (i.e. genomovars) based upon DNA--DNA similarities. The internally transcribed 16S--23S rDNA spacer (ITS1) regions of P. stutzeri were analysed with respect to the ability of these nucleic acid regions to differentiate and identify the genomic groups (i.e. genomovars) of P. stutzeri. The ITS1s of 34 strains of P. stutzeri were amplified by PCR and the PCR product was subjected to RFLP analysis, which allowed the differentiation and identification of the strains to their respective genomovars. Sequence determination and analysis of ITS1s supported further the results obtained by RFLP, i.e. nucleotide signatures were identified in strains belonging to different genomovars. The ITS1s of all strains of P. stutzeri contained the tandem tRNA(Ile)/tRNA(Ala) genes and did not exhibit distinct sequence heterogeneity between different operons of a strain. Phylogenetically informative variable sites were located, exclusively, in non-coding regions. The results of the RFLP and sequence analysis of ITS1s supported and correlated with the phylogenetic relationships estimated from 16S rRNA gene sequence comparisons and DNA--DNA hybridizations, offering an alternative tool for genomovar and species differentiation.
This article has been cited by other articles:
|
|
 |
|
  M. Gomila, B. Bowien, E. Falsen, E. R. B. Moore, and J. Lalucat Description of Pelomonas aquatica sp. nov. and Pelomonas puraquae sp. nov., isolated from industrial and haemodialysis water Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2629 - 2635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Bent, J. D. Pierson, L. J. Forney, R. Danovaro, G. M. Luna, A. Dell'Anno, and B. Pietrangeli Measuring Species Richness Based on Microbial Community Fingerprints: the Emperor Has No Clothes Appl. Envir. Microbiol., April 1, 2007; 73(7): 2399 - 2401. [Full Text] [PDF]
|
|
|
|
|
|
R. Danovaro, G. M. Luna, A. Dell'Anno, and B. Pietrangeli Comparison of Two Fingerprinting Techniques, Terminal Restriction Fragment Length Polymorphism and Automated Ribosomal Intergenic Spacer Analysis, for Determination of Bacterial Diversity in Aquatic Environments Appl. Envir. Microbiol., September 1, 2006; 72(9): 5982 - 5989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lalucat, A. Bennasar, R. Bosch, E. Garcia-Valdes, and N. J. Palleroni Biology of Pseudomonas stutzeri Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 510 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sikorski, J. Lalucat, and W. Wackernagel Genomovars 11 to 18 of Pseudomonas stutzeri, identified among isolates from soil and marine sediment Int J Syst Evol Microbiol, September 1, 2005; 55(5): 1767 - 1770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. C. Chang, Y. F. Wei, L. Dijkshoorn, M. Vaneechoutte, C. T. Tang, and T. C. Chang Species-Level Identification of Isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii Complex by Sequence Analysis of the 16S-23S rRNA Gene Spacer Region J. Clin. Microbiol., April 1, 2005; 43(4): 1632 - 1639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Conrads, D. M. Citron, K. L. Tyrrell, H.-P. Horz, and E. J. C. Goldstein 16S-23S rRNA gene internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus Porphyromonas Int J Syst Evol Microbiol, March 1, 2005; 55(2): 607 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Cladera, A. Bennasar, M. Barcelo, J. Lalucat, and E. Garcia-Valdes Comparative Genetic Diversity of Pseudomonas stutzeri Genomovars, Clonal Structure, and Phylogeny of the Species J. Bacteriol., August 15, 2004; 186(16): 5239 - 5248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-R. Abraham, C. Strompl, M. Vancanneyt, A. Bennasar, J. Swings, H. Lunsdorf, J. Smit, and E. R. B. Moore Woodsholea maritima gen. nov., sp. nov., a marine bacterium with a low diversity of polar lipids Int J Syst Evol Microbiol, July 1, 2004; 54(4): 1227 - 1234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Holmes, M. P. Holley, A. Mahon, B. Nield, M. Gillings, and H. W. Stokes Recombination Activity of a Distinctive Integron-Gene Cassette System Associated with Pseudomonas stutzeri Populations in Soil J. Bacteriol., February 1, 2003; 185(3): 918 - 928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fernandez-Martinez, M. J. Pujalte, J. Garcia-Martinez, M. Mata, E. Garay, and F. Rodriguez-Valera Description of Alcanivorax venustensis sp. nov. and reclassification of Fundibacter jadensis DSM 12178T (Bruns and Berthe-Corti 1999) as Alcanivorax jadensis comb. nov., members of the emended genus Alcanivorax Int J Syst Evol Microbiol, January 1, 2003; 53(1): 331 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. K. Toth, A. O. Avrova, and L. J. Hyman Rapid Identification and Differentiation of the Soft Rot Erwinias by 16S-23S Intergenic Transcribed Spacer-PCR and Restriction Fragment Length Polymorphism Analyses Appl. Envir. Microbiol., September 1, 2001; 67(9): 4070 - 4076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Rius, M. C. Fusté, C. Guasp, J. Lalucat, and J. G. Lorén Clonal Population Structure of Pseudomonas stutzeri, a Species with Exceptional Genetic Diversity J. Bacteriol., January 15, 2001; 183(2): 736 - 744. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
