International Journal of Systematic and Evolutionary Microbiology, Vol 50, 1259-1264, Copyright © 2000 by Society for General Microbiology

Clostridium peptidivorans sp. nov., a peptide-fermenting bacterium from an olive mill wastewater treatment digester

T Mechichi, ML Fardeau, M Labat, JL Garcia, F Verhe and BKC Patel
Laboratoire IRD de Microbiologie, Universite de Provence, CESB/ESIL, Case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France

A new peptide-degrading, strictly anaerobic bacterium, designated strain TMC4(T), was isolated from an olive mill wastewater treatment digester. Cells of strain TMC4(T) were motile, rod-shaped (5--10 x 0.6--1.2 microm), stained Gram-positive and formed terminal to subterminal spores that distended the cells. Optimal growth occurred at 37 degrees C and pH 7 in an anaerobic basal medium containing 0.5% Casamino acids. Arginine, lysine, cysteine, methionine, histidine, serine, isoleucine, yeast extract, peptone, Biotrypcase, gelatin and crotonate also supported growth, but not carbohydrates, organic acids or alcohols. The end-products of degradation were: acetate and butyrate from lysine and crotonate; acetate, butyrate, H(2) and CO(2) from Biotrypcase, gelatin and peptone; acetate, alanine, H(2) and CO(2) from cysteine; acetate, H(2) and CO(2) from serine, cysteine and yeast extract; acetate and formate from histidine; propionate from methionine; methyl 2-butyrate, H(2) and CO(2) from isoleucine; acetate and ethanol from arginine; and acetate, propionate, butyrate, methyl 2-butyrate, H(2) and CO(2) from Casamino acids. The DNA G+C content of strain TMC4(T) was 31 mol%. Phylogeny based on 16S rRNA sequence analysis showed that strain TMC4(T) was a member of the low-G+C-content Gram-positive genus Clostridium, with the closest relative being Clostridium pascui (sequence similarity of 96 %). Due to considerable differences in genomic and phenotypic properties between strain TMC4(T) and those of its nearest relative, strain TMC4(T) is proposed as a new species of the genus Clostridium, Clostridium peptidivorans sp. nov. Strain TMC4(T) has been deposited in the DSMZ as strain DSM 12505(T).

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