International Journal of Systematic and Evolutionary Microbiology, Vol 50, 1187-1191, Copyright © 2000 by Society for General Microbiology

Sequence analysis of domains III and IV of the 23S rRNA gene of verticillate streptomycetes

S Palmano, G Firrao and R Locci
Dipartimento di Biologia Applicata alla Difesa delle Piante, Universita di Udine, via delle Scienze 208, 33100 Udine, Italy

Domains III and IV of the 23S rRNA gene of 25 strains of verticillate streptomycetes were sequenced. None of the sequences was identical to any other, with regions of variability being restricted to parts of helices 54 and 64. No relationships were detected between the similarities in the sequence and the assignment to phenetic clusters as defined by the numerical taxonomy studies. Limited agreement was also found between similarity of the sequences and DNA--DNA homology values. However, species (< 70% DNA--DNA homology values)-specific diagnostic oligonucleotides generally could be defined, except for Streptomyces baldaccii. Therefore the determination of the 23S rRNA sequence may be of greater value for fingerprinting individual strains than for taxonomic or identification purposes.

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