International Journal of Systematic and Evolutionary Microbiology, Vol 50, 639-644, Copyright © 2000 by Society for General Microbiology
Desulfovirga adipica gen. nov., sp. nov., an adipate-degrading, Gram-negative, sulfate-reducing bacterium
K Tanaka, E Stackebrandt, S Tohyama and T Eguchi
Applied Microbiology Department, National Institute of Bioscience and Human-Technology, Higashi 1-1, Tsukuba, Ibaraki 305-8566, Japan
A novel, mesophilic, Gram-negative bacterium was isolated from an anaerobic
digestor for municipal wastewater. The bacterium degraded adipate in the
presence of sulfate, sulfite, thiosulfate and elemental sulfur.
(E)-2-Hexenedioate accumulated transiently in the degradation of adipate.
(E)-2-Hexenedioate, (E)-3-hexenedioate, pyruvate, lactate, C(1)--C(12)
straight-chain fatty acids and C(2)--C(10) straight-chain primary alcohols
were also utilized as electron donors. 3-Phenylpropionate was oxidized to
benzoate. The G+C content of the DNA was 60 mol%. 16S rDNA sequence
analysis revealed that the new isolate clustered with species of the genus
Syntrophobacter and Desulforhabdus amnigenus. Strain TsuAS1(T) resembles
Desulforhabdus amnigenus DSM 10338(T) with respect to the ability to
utilize acetate as an electron donor and the inability to utilize
propionate without sulfate in co-culture with Methanospirillum hungatei DSM
864. Strains TsuAS1(T) and DSM 10338(T) form a 'non-syntrophic subcluster'
within the genus Syntrophobacter. Desulfovirga adipica gen. nov., sp. nov.
is proposed for the newly isolated bacterium, with strain TsuAS1(T) (=DSM
12016(T)) as the type strain.
