IJSEM Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Birtles, R. J.
Right arrow Articles by Raoult, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Birtles, R. J.
Right arrow Articles by Raoult, D.
Agricola
Right arrow Articles by Birtles, R. J.
Right arrow Articles by Raoult, D.

International Journal of Systematic and Evolutionary Microbiology, Vol 50, 63-72, Copyright © 2000 by International Union of Microbiological Societies

ARTICLES

'Candidatus Odyssella thessalonicensis' gen. nov., sp. nov., an obligate intracellular parasite of Acanthamoeba species

RJ Birtles, TJ Rowbotham, R Michel, DG Pitcher, B Lascola, S Alexiou-Daniel and D Raoult
Unite des Rickettsies, CNRS UPRESA 6020, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille cedex 5, France

An intracellular bacterium, strain L13, was observed infecting an environmental isolate of an Acanthamoeba species. The bacterium could not be recovered on axenic medium but was recovered and cultivated in vitro using cultures of Acanthamoeba polyphaga. The 16S rRNA gene sequence of L13 was found to be new, sharing less than 84% similarity with other sequences in the GenBank/EMBL database. L13 was found to be a member of the alpha-Proteobacteria, sharing an evolutionary line of descent with a group of uniquely obligate intracellular organisms comprised of Caedibacter and Holospora species and the NHP bacterium. Viable bacteria appeared to be highly motile within amoebae. Ultrastructural analysis of the bacterium demonstrated that it is rod-shaped and possesses a typical Gram-negative cell wall, but has no other outstanding features except small vesicle-like structures often associated with the outer surface of each bacterium. The host range of L13 was found to be limited to the genus Acanthamoeba. In A. polyphaga, L13 infection was slow to manifest when cultures were incubated below 30 degrees C, but at higher temperatures bacteria multiplied prolifically and induced host cell lysis. The protein profile of the bacterium purified from the amoebae was assessed by SDS-PAGE and its G+C content was estimated to be 41 mol%. Although these results support the proposal of L13 as a new species, its obligate intracellular nature prevented isolation of a definitive type strain. L13 is therefore proposed as 'Candidatus Odyssella thessalonicensis' gen. nov., sp. nov.


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
A. Ohno, N. Kato, R. Sakamoto, S. Kimura, and K. Yamaguchi
Temperature-Dependent Parasitic Relationship between Legionella pneumophila and a Free-Living Amoeba (Acanthamoeba castellanii)
Appl. Envir. Microbiol., July 15, 2008; 74(14): 4585 - 4588.
[Abstract] [Full Text] [PDF]

Home page
 Int. J. Syst. Evol. Microbiol.Home page
K.-B. Lee, C.-T. Liu, Y. Anzai, H. Kim, T. Aono, and H. Oyaizu
The hierarchical system of the 'Alphaproteobacteria': description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.
Int J Syst Evol Microbiol, September 1, 2005; 55(5): 1907 - 1919.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
M. Molmeret, M. Horn, M. Wagner, M. Santic, and Y. Abu Kwaik
Amoebae as Training Grounds for Intracellular Bacterial Pathogens
Appl. Envir. Microbiol., January 1, 2005; 71(1): 20 - 28.
[Full Text] [PDF]

Home page
 MicrobiologyHome page
A. Collingro, S. Poppert, E. Heinz, S. Schmitz-Esser, A. Essig, M. Schweikert, M. Wagner, and M. Horn
Recovery of an environmental chlamydia strain from activated sludge by co-cultivation with Acanthamoeba sp.
Microbiology, January 1, 2005; 151(1): 301 - 309.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
G. Greub and D. Raoult
Microorganisms Resistant to Free-Living Amoebae
Clin. Microbiol. Rev., April 1, 2004; 17(2): 413 - 433.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
B. J. Baker, P. Hugenholtz, S. C. Dawson, and J. F. Banfield
Extremely Acidophilic Protists from Acid Mine Drainage Host Rickettsiales-Lineage Endosymbionts That Have Intervening Sequences in Their 16S rRNA Genes
Appl. Envir. Microbiol., September 1, 2003; 69(9): 5512 - 5518.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
F. Marciano-Cabral and G. Cabral
Acanthamoeba spp. as Agents of Disease in Humans
Clin. Microbiol. Rev., April 1, 2003; 16(2): 273 - 307.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
C. L. Beier, M. Horn, R. Michel, M. Schweikert, H.-D. Gortz, and M. Wagner
The Genus Caedibacter Comprises Endosymbionts of Paramecium spp. Related to the Rickettsiales (Alphaproteobacteria) and to Francisella tularensis (Gammaproteobacteria)
Appl. Envir. Microbiol., December 1, 2002; 68(12): 6043 - 6050.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2000 by the International Union of Microbiological Societies.