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Sporobacterium olearium gen. nov., sp. nov., a new methanethiol-producing bacterium that degrades aromatic compounds, isolated from an olive mill wastewater treatment digester

¹ Laboratoire ORSTOM de Microbiologie des Anaérobies, Université de Provence, CESB-ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
² Departement de Biologie, Université de la Méditerranée, 13288 Marseille Cedex 9, France
³ School of Biomolecular and Biomedical Sciences, Faculty of Science, Griffith University, Brisbane, Queensland 4111, Australia

Author for correspondence: Bharat K. C. Patel. Tel: +61 417 726 671. Fax: +61 7 3875 7656. e-mail: bharat{at}genomes.sci.gu.edu.au

ABSTRACT

A strictly chemo-organotrophic, anaerobic bacterium was isolated from an olive mill wastewater treatment digester on syringate and designated strain SR1^T. The cells were slightly curved rods, stained Gram-positive and possessed terminal spores. Strain SR1^T utilized crotonate, methanol and a wide range of aromatic compounds including 3,4,5-trimethoxybenzoate (TMB), 3,4,5-trimethoxycinnamate (TMC), syringate, 3,4,5-trimethoxyphenylacetate (TMPA), 3,4,5-trimethoxyphenylpropionate (TMPP), ferulate, sinapate, vanillate, 3,4-dimethoxybenzoate, 2,3-dimethoxybenzoate, gallate, 2,4,6-trihydroxybenzoate (THB), pyrogallol, phloroglucinol and quercetin as carbon and energy sources. Acetate and butyrate were produced from aromatic compounds, methanol and crotonate whereas methanethiol (MT) was produced from methoxylated aromatic compounds and methanol. Strain SR1^T had a G+C content of 38 mol% and grew optimally between 37 and 40 °C at pH 7·2 on a crotonate-containing medium. Phylogenetically, strain SR1^T was a member of cluster XIVa of the Clostridiales group and shared a sequence similarity of 90% with Clostridium aminovalericum and Eubacterium fissicatena. Consequently, its precise neighbourliness to any one of them depended on the selection of strains of the cluster. On the basis of the phylogenetic and phenotypic evidence presented in this paper, the designation of strain SR1^T as Sporobacterium olearium gen. nov., sp. nov. is proposed. The type strain is SR1^T (= DSM 12504^T).

Key Words: Sporobacterium olearium • anaerobic degradation • aromatic compounds • methanethiol • ring cleavage

The GenBank accession number for the 16S rRNA sequence of strain SR1^T is AF116854.

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