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Phylogenetic analysis of genus Marinilabilia and related bacteria based on the amino acid sequences of GyrB and emended description of Marinilabilia salmonicolor with Marinilabilia agarovorans as its subjective synonym

Makoto Suzuki^1,, Yasuyoshi Nakagawa², Shigeaki Harayama¹ and Satoshi Yamamoto¹

¹ Marine Biotechnology Institute, Kamaishi Laboratories, Kamaishi, Iwate 026–0001, Japan
² Institute for Fermentation, Osaka, 2–17–85 Jyuso-Honcho, Yodogawa-ku, Osaka 532–0024, Japan

Author for correspondence: Makoto Suzuki. Tel: +81 42 725 2555. Fax: +81 42 726 8330. e-mail: makoto.suzuki{at}kyowa.co.jp

ABSTRACT

The detailed phylogenetic relationships for genus Marinilabilia and related taxa were analysed by using DNA gyrase B subunit gene (gyrB) sequences. Anaerobic bacteria in the Cytophaga-Flavobacterium-Bacteroides phylum, namely genera Marinilabilia, Bacteroides, Rikenella, Prevotella and Porphyromonas and Cytophaga fermentans, were clustered in the same branch and the facultative anaerobes Marinilabilia and Cytophaga fermentans formeed a subcluster in the branch of the anaerobic bacteria. Phylogenetic analysis using 16S rDNA sequences gave a similar result but with a lower bootstrap value for each cluster. The gyrB sequences of Marinilabilia salmonicolor and Marinilabilia agarovorans were the same, and the relatedness of their chromosomal DNA, as determined by DNA-DNA hybridization, was greater than 70%. These genetic aspects led to the conclusion that M. salmonicolor IFO 15948^T and M. agarovorans IFO 14957^T belong to a single species. Since M. salmonicolor was described first, as Cytophaga salmonicolor, M. salmonicolor is a senior subjective synonym of M. agarovorans. Therefore, the name M. salmonicolor should be retained and strain IFO 14957^T should be reclassified as M. salmonicolor. However, the agar-degrading ability of strain IFO 14957^T is a prominent biochemical characteristic. It is therefore proposed that strain IFO 14957^T should be renamed M. salmonicolor biovar agarovorans.

Key Words: Marinilabilia • Cytophaga-Flavobacterium-Bacteroides complex • gyrB • phylogeny

Present address: Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd, 3–6–6 Asahi-machi, Machida-shi, Tokyo 194–8533, Japan.

The DDBJ accession numbers for the gyrB sequences reported in this paper are AB15029 (IFO 14291^T), AB15026 (ATCC 43767^T), AB15024(IAM 14198^T), AB15025 (IFO 14944^T), AB15038 (IFO 16043^T), AB15037 (IAM 12607^T), AB15028 (IFO 14943^T), AB15032 (IFO 15948^T), AB15033 (IFO 14957^T), AB15036 (IAM 14210^T), AB15035 (IFO 12017^T), AB15027 (ATCC 43766^T), AB15030 (JCM 6326^T), AB15031 (IFO 15936^T) and AB15034 (JCM 2053^T).

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